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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
Ec holE-pCDFDuet
 
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RRID:Addgene_241264 holE E. coli Streptomycin Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:33:43 0
Sc hk/P-RFA3-pCDFDuet
 
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1+ mentions
RRID:Addgene_240743 hk/P-RFA3 Saccharomyces cerevisiae Streptomycin Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:33:42 2
pENTR223-ABL2(K281M)
 
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RRID:Addgene_215468 ABL2 Homo sapiens Streptomycin PMID:40419795 Backbone Marker:Thermo Fisher Scientific Inc.; Backbone Size:4761; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Streptomycin K281M (kinase-inactivating) 2026-08-15 01:33:49 0
pENTR223-ABL2(WT)
 
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RRID:Addgene_215467 ABL2 Homo sapiens Streptomycin PMID:40419795 Backbone Marker:Invitrogen; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Streptomycin Includes stop codon 2026-08-15 01:33:49 0
Hv_HPT:pCSRM2C:Gus_introns
 
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RRID:Addgene_215191 Scream2 Other Streptomycin PMID:39943924 Backbone Marker:Sylvestre Marillonnet; Backbone Size:5201; Vector Backbone:pAGM8031; Vector Types:; Bacterial Resistance:Streptomycin 2026-08-15 01:32:56 0
HE_150
 
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RRID:Addgene_201051 n/a Streptomycin This strain has 1059 genetic changes compared to the DH10B reference genome in Genbank (CP000948.1). Please see https://www.ncbi.nlm.nih.gov/nuccore/CP110015 and the accompanying paper for more details. Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Streptomycin 2026-08-15 01:26:30 0
HE_100
 
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RRID:Addgene_201050 n/a Streptomycin This strain has 797 genetic changes compared to the DH10B reference genome in Genbank (CP000948.1). Please see https://www.ncbi.nlm.nih.gov/nuccore/CP110016 and the accompanying paper for more details. Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Streptomycin 2026-08-15 01:26:30 0
pIVM_26
 
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1+ mentions
RRID:Addgene_204501 EloB (1-104) and EloC (17-112) Homo sapiens Streptomycin PMID:28591624 Gadd MS, Testa A, Lucas X, Chan KH, Chen W, Lamont DJ, Zengerle M, Ciulli A. Structural basis of PROTAC cooperative recognition for selective protein degradation. Nat Chem Biol. 2017 May;13(5):514-521. doi: 10.1038/nchembio.2329. Epub 2017 Mar 13. PMID: 28288108; PMCID: PMC5392356. Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDF-1b DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 0 2026-08-15 01:27:44 3
pCDF_TypeIC_target_sfGFP_invivo
 
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RRID:Addgene_196399 type IC dsDNA target Synthetic Streptomycin PMID:36805026 Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:27:30 0
HB101 endA::frt
 
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RRID:Addgene_45473 endA::frt Streptomycin PMID:21306445 endA was deleted for cleaner and higher yield plasmid preps. Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin major endonuclease endA deleted 2026-08-15 01:15:24 0
pTDpelB-CTwinStrep
 
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RRID:Addgene_45941 Streptomycin PMID:23687945 This plasmid was tested in the Gram-negative soil bacterium Pseudomonas putida KT2440 and Escherichia coli K12 and is especially suited for protein production, affinity purification, protein complex copurification with SPINE (Strep Protein Interaction Experiments) or (co-)localization studies. Due to the broad host range of the RK2 origin of replication, the plasmid facilitates experimental verification of hypothetical proteins and protein production yield assessment in different expression hosts possibly including new isolates. The Supplementary Table S1 in the following publication lists approximately 30 strains in which the RK2 origin of replication should be functional. Silva-Rocha et al., The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Research 2013, 41:D666-675. http://nar.oxfordjournals.org/content/41/D1/D666.long Backbone Marker:SEVA (de Lorenzo Lab); Vector Backbone:pSEVA424; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:15:27 0
pTD-C_sfYFPTwinStrep
 
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RRID:Addgene_45943 synthetic sfYFP (codon usage adapted to P.putida KT2440) Aequorea victoria Streptomycin PMID:23687945 This plasmid was tested in the Gram-negative soil bacterium Pseudomonas putida KT2440 and Escherichia coli K12 and is especially suited for protein production, affinity purification, protein complex copurification with SPINE (Strep Protein Interaction Experiments) or (co-)localization studies. Due to the broad host range of the RK2 origin of replication, the plasmid facilitates experimental verification of hypothetical proteins and protein production yield assessment in different expression hosts possibly including new isolates. The Supplementary Table S1 in the following publication lists approximately 30 strains in which the RK2 origin of replication should be functional. Silva-Rocha et al., The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Research 2013, 41:D666-675. http://nar.oxfordjournals.org/content/41/D1/D666.long Backbone Marker:Dammeyer et al. 2013; Vector Backbone:pTD-CTwinStrep; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:15:27 0
pTD-C_eYFPTwinStrep
 
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RRID:Addgene_45942 synthetic eYFP (codon usage adapted to E.coli K12) Aequorea victoria Streptomycin PMID:23687945 This plasmid was tested in the Gram-negative soil bacterium Pseudomonas putida KT2440 and Escherichia coli K12 and is especially suited for protein production, affinity purification, protein complex copurification with SPINE (Strep Protein Interaction Experiments) or (co-)localization studies. Due to the broad host range of the RK2 origin of replication, the plasmid facilitates experimental verification of hypothetical proteins and protein production yield assessment in different expression hosts possibly including new isolates. The Supplementary Table S1 in the following publication lists approximately 30 strains in which the RK2 origin of replication should be functional. Silva-Rocha et al., The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Research 2013, 41:D666-675. http://nar.oxfordjournals.org/content/41/D1/D666.long Backbone Marker:Dammeyer et al. 2013; Vector Backbone:pTD-CTwinStrep; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:15:27 0
pAF-HdrB3
 
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RRID:Addgene_184908 Cas9-sgRNA-homology arms Synthetic Streptomycin PMID:37556825 Please visit https://www.biorxiv.org/content/10.1101/2022.03.14.484339v1 for bioRxiv preprint. Vector Backbone:pJRD215; Vector Types:Bacterial Expression, CRISPR, broad-host-range; Bacterial Resistance:Streptomycin 2026-08-15 01:23:41 0
pREDawn-StrR-MCS
 
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RRID:Addgene_188979 Streptomycin PMID:35998606 Please note that Addgene's NGS found a 65bp insertion in the ori region as compared to the Depositor's sequence. The depositor has confirmed that the plasmid functions as described in the associated publication. Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:23:47 0
pYI048
 
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RRID:Addgene_170844 pGH3.17::SMT2-FLAG Streptomycin PMID:36216814 Please visit https://doi.org/10.1101/2021.06.16.448628 for bioRxiv preprint. Vector Backbone:pYI001; Vector Types:Plant Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:23:57 0
pYI046
 
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RRID:Addgene_170842 pUBQ10::SMT2-FLAG::tOCS Streptomycin PMID:36216814 Please visit https://doi.org/10.1101/2021.06.16.448628 for bioRxiv preprint. Vector Backbone:pFP100; Vector Types:Plant Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:23:57 0
pYI047
 
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RRID:Addgene_170843 pFRO6::SMT2-FLAG Streptomycin PMID:36216814 Please note that the Addgene verified sequence differs from the depositor reference sequence linked in the supplemental documents section. These differences did not impact plasmid function. The primers 5' - ATCCAAGCTCAAGCTAAGCTcgatgctctcaaggccaa and 3' -TATCTCATTAAAGCAGGATCCTCACTTGTCATCGTCGTCCTTGTAATCAGAACTCTCCTCCGGT were previously used, although the 5' primer differs slightly from the Addgene verified sequence. Please visit https://doi.org/10.1101/2021.06.16.448628 for bioRxiv preprint. Vector Backbone:pYI001; Vector Types:Plant Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:24:00 0
pYI012
 
Resource Report
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RRID:Addgene_170841 GH3.17p Streptomycin PMID:36216814 Please visit https://doi.org/10.1101/2021.06.16.448628 for bioRxiv preprint. Vector Backbone:pYI001; Vector Types:Plant Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:23:57 0
NCH1exp
 
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RRID:Addgene_131017 NCH1 Zea mays (maize) Streptomycin PMID:33679862 Backbone Size:11546; Vector Backbone:pH7WG2; Vector Types:Plant Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:22:54 0

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