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Species: Synthetic
Genetic Insert: MCS-P2A-EGFP
Vector Backbone Description: Backbone Marker:Vector Builder; Backbone Size:4643; Vector Backbone:pAAV-SynI; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34515305
Proper citation: RRID:Addgene_176276 Copy
Species: Synthetic
Genetic Insert: HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
Vector Backbone Description: Vector Backbone:piggyBac-EF1alpha; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34403072
Comments: Plasmid name in Yang lab: HGpl553
Proper citation: RRID:Addgene_176459 Copy
Species: Synthetic
Genetic Insert: HNHx-ABEmax7.10HD
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34631280
Proper citation: RRID:Addgene_176477 Copy
Species: Synthetic
Genetic Insert: BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
Vector Backbone Description: Vector Backbone:piggyBac-EF1alpha; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34403072
Comments: Plasmid name in Yang lab: HGpl268
Proper citation: RRID:Addgene_176461 Copy
Species: Synthetic
Genetic Insert: CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
Vector Backbone Description: Backbone Marker:Addgene 171992; Vector Backbone:PEA1-Nuclease-Puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34534334
Proper citation: RRID:Addgene_176528 Copy
Species: Synthetic
Genetic Insert: CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
Vector Backbone Description: Backbone Marker:Addgene 171991; Vector Backbone:PEA1-Puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34534334
Proper citation: RRID:Addgene_176527 Copy
Species: Synthetic
Genetic Insert: I-SceI sites, Tol2 repeats and Tn5-neo cassette
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:34751773
Proper citation: RRID:Addgene_176619 Copy
Species: Synthetic
Genetic Insert: TagRFP657
Vector Backbone Description: Vector Backbone:pM; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35314781
Comments: Please note: Plasmid contains E38G and L304S mutations in MET17. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_176563 Copy
Species: Synthetic
Genetic Insert: TagRFP657
Vector Backbone Description: Vector Backbone:pH; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35314781
Proper citation: RRID:Addgene_176560 Copy
Species: Synthetic
Genetic Insert: EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:PiggyBac transposon system; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35050677
Proper citation: RRID:Addgene_176630 Copy
Species: Synthetic
Genetic Insert: TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:PiggyBac transposon system; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35050677
Proper citation: RRID:Addgene_176626 Copy
Species: Synthetic
Genetic Insert: 14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:PiggyBac transposon system; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35050677
Proper citation: RRID:Addgene_176629 Copy
Species: Synthetic
Genetic Insert: 14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:PiggyBac transposon system; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35050677
Proper citation: RRID:Addgene_176628 Copy
Species: Synthetic
Genetic Insert: N-terminus of PE2
Vector Backbone Description: Backbone Size:3544; Vector Backbone:px601; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34446856
Proper citation: RRID:Addgene_176649 Copy
Species: Synthetic
Genetic Insert: MBP-RGG-LgBit-RGG
Vector Backbone Description: Vector Backbone:pBamUK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34648259
Proper citation: RRID:Addgene_176643 Copy
Species: Synthetic
Genetic Insert: eCFP
Vector Backbone Description: Vector Backbone:pK; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31367038
Proper citation: RRID:Addgene_176559 Copy
Species: Synthetic
Genetic Insert: eCFP
Vector Backbone Description: Vector Backbone:pM; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35314781
Proper citation: RRID:Addgene_176558 Copy
Species: Synthetic
Genetic Insert: PE2-NG variant
Vector Backbone Description: Vector Backbone:pLenti-PE2-BSD; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34446856
Proper citation: RRID:Addgene_176933 Copy
Species: Synthetic
Genetic Insert: moxGFP-P2A-T2A
Vector Backbone Description: Backbone Marker:Eric Kowarz; Backbone Size:5857; Vector Backbone:pSBbi-Pur (Addgene Plasmid #60523); Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34877484
Comments: The moxGFP-P2A-T2A construct was designed by Jorge L. Arias-Arias from Universidad de Costa Rica ([email protected]). It was codon optimized for human and mouse expression and includes two additional cloning sites (5' EcoRV - 3' SalI and 5' SmaI - 3' XbaI, see the map uploaded by the depositor) for the polycistronic expression of up to three genes: moxGFP and two more. This vector is suitable for transient transfection, but it was intended to be co-transfected with the transposase vector pCMV(CAT)T7-SB100 (Addgene Plasmid #34879) in order to generate mammalian cells with stable expression of the genes of interest. Enjoy!
Proper citation: RRID:Addgene_176893 Copy
Species: Synthetic
Genetic Insert: yeGFP
Vector Backbone Description: Backbone Marker:EUROSCARF; Backbone Size:6091; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34939781
Comments: This plasmid was used as a template to amplify the reporter cassette for the genomic integration.
Proper citation: RRID:Addgene_177163 Copy
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