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Vector Backbone Description: Vector Backbone:pFE604T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:38724726
Proper citation: RRID:Addgene_202601 Copy
Vector Backbone Description: Backbone Marker:Spaink; Backbone Size:10400; Vector Backbone:pMP103; Vector Types:Bacterial Expression, IncP; Bacterial Resistance:Tetracycline
Defining Citation: PMID:24276795
Comments: the polylinker of pIC20H is broadly available and isn't covered by an MTA
Proper citation: RRID:Addgene_185921 Copy
Species: Synthetic
Genetic Insert: lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
Vector Backbone Description: Vector Backbone:pFW11 Tet; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37406096
Proper citation: RRID:Addgene_199119 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_205019 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_205017 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pMflT-o4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Comments: The user should always streak the culture on plate and work with isolated fluorescent colonies. Addgene NGS results found mixed population of nucleotides across Term_apFAB390 and C-terminus of NeonGreen, but plasmid function should not be affected.
Proper citation: RRID:Addgene_201855 Copy
Vector Backbone Description: Backbone Marker:Host-Microbe Interactions lab; Backbone Size:2437; Vector Backbone:pUdO1te; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:38235654
Proper citation: RRID:Addgene_210242 Copy
Vector Backbone Description: Backbone Marker:Host-Microbe Interactions lab; Backbone Size:3241; Vector Backbone:pUdO4te; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:38235654
Proper citation: RRID:Addgene_210245 Copy
Species: Synthetic
Genetic Insert: OsU6.2-sgRNA7 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199733 Copy
Species: Synthetic
Genetic Insert: TaU6-sgRNA8 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199734 Copy
Species: Synthetic
Genetic Insert: ZmU6C3-sgRNA5 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199731 Copy
Species: Synthetic
Genetic Insert: ZmU6C1-sgRNA4 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199730 Copy
Species: Synthetic
Genetic Insert: OsU3-sgRNA2 scaffold
Vector Backbone Description: Vector Backbone:pYPQ132D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199728 Copy
Species: Synthetic
Genetic Insert: TaU3-sgRNA3 scaffold
Vector Backbone Description: Vector Backbone:pYPQ131D; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199729 Copy
Vector Backbone Description: Vector Backbone:pCBLsgRNA2; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199737 Copy
Species: S. meliloti
Genetic Insert: exoY
Vector Backbone Description: Backbone Marker:Kay Jones; Vector Backbone:pstb-LAFR5; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:22685282
Proper citation: RRID:Addgene_86170 Copy
Species: Methanocaldococcus jannaschii
Genetic Insert: para-cyanophenylalanine Mj synthetase
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:19492814
Proper citation: RRID:Addgene_85494 Copy
Species: Methanocaldococcus jannaschii
Genetic Insert: 3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:24611875
Proper citation: RRID:Addgene_85498 Copy
Species: Methanocaldococcus jannaschii
Genetic Insert: IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:20839808
Comments: This synthetase is permissive and can incorporate a variety of amino acids structurally analogous to IBBN. For a more complete functional and structural analysis of this synthetase, also see Cooley et al. Chembiochem. 2014, 15(12): 1810–1819.
Proper citation: RRID:Addgene_85500 Copy
Species: Methanocaldococcus jannaschii
Genetic Insert: tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:20082521
Proper citation: RRID:Addgene_85484 Copy
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