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Species: Saccharomyces cerevisiae
Genetic Insert: His/Vps27(345-440) – Hse1(275-375)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pST39; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17543868
Proper citation: RRID:Addgene_17638 Copy
Genetic Insert: PspCas13b
Vector Backbone Description: Vector Backbone:pUb-3xFLAG-MCS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35244715
Proper citation: RRID:Addgene_176306 Copy
Genetic Insert: PspCas13b
Vector Backbone Description: Vector Backbone:pUb-3xFLAG-MCS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35244715
Comments: Depositor confirms E113K, N265S mutations in PspCas13b does not affect plasmid function.
Proper citation: RRID:Addgene_176305 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:Hy_pUbi-p63e eGFP SV40; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35244715
Proper citation: RRID:Addgene_176300 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:Hy_pMtnA eGFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35244715
Proper citation: RRID:Addgene_176302 Copy
Genetic Insert: RxCas13d
Vector Backbone Description: Vector Backbone:pUb-3xFLAG-MCS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35244715
Proper citation: RRID:Addgene_176304 Copy
Genetic Insert: RxCas13d
Vector Backbone Description: Vector Backbone:pUb-3xFLAG-MCS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35244715
Proper citation: RRID:Addgene_176303 Copy
Species: Homo sapiens
Genetic Insert: ZNF608 gRNA
Vector Backbone Description: Backbone Marker:addgene ID: 42230; Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed also includes (uncloned) PAM
Proper citation: RRID:Addgene_176380 Copy
Species: Homo sapiens
Genetic Insert: SOX2 gRNA
Vector Backbone Description: Backbone Marker:addgene ID: 42230; Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed also includes (uncloned) PAM
Proper citation: RRID:Addgene_176382 Copy
Species: Synthetic
Genetic Insert: HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
Vector Backbone Description: Vector Backbone:piggyBac-EF1alpha; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34403072
Comments: Plasmid name in Yang lab: HGpl553
Proper citation: RRID:Addgene_176459 Copy
Genetic Insert: SARS-CoV-2-RBD-SD1-L452R
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176452 Copy
Genetic Insert: SARS-CoV-2-RBD-SD1-S477N
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176453 Copy
Genetic Insert: SARS-CoV-2-RBD-SD1-L452R-T478K
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176456 Copy
Genetic Insert: SARS-CoV-2-RBD-SD1-L452R-E484Q
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176455 Copy
Genetic Insert: SARS-CoV-2-RBD-SD1-WA1
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176448 Copy
Genetic Insert: SARS-CoV-2-RBD-K417N-E484K-N501Y
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176440 Copy
Genetic Insert: SARS-CoV-2-RBD-S477N
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176443 Copy
Genetic Insert: SARS-CoV-2-RBD-L452R-T478K
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176446 Copy
Species: Mus musculus
Genetic Insert: hU6-sgCRY1-2
Vector Backbone Description: Backbone Size:5288; Vector Backbone:pAAV-hU6-gRNA-hSyn-mCherry-KASH; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33510438
Comments: Please note that the mutations K14E, N210D, and E249G were found in the mCherry sequence. The fluorophore is still fluorescent, as described in the publication.
Proper citation: RRID:Addgene_176511 Copy
Species: Synthetic
Genetic Insert: HNHx-ABEmax7.10HD
Vector Backbone Description: Vector Backbone:p2T-CMV-BlastR; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34631280
Proper citation: RRID:Addgene_176477 Copy
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