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Species: Homo sapiens
Genetic Insert: CDX4
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: WTC11 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176343 Copy
Species: Homo sapiens
Genetic Insert: NPAS1
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176346 Copy
Species: Homo sapiens
Genetic Insert: CGGBP1
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176345 Copy
Species: Homo sapiens
Genetic Insert: THAP12
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176348 Copy
Species: Homo sapiens
Genetic Insert: DNAJC1
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176340 Copy
Species: Homo sapiens
Genetic Insert: ZNF79
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176371 Copy
Species: Homo sapiens
Genetic Insert: SOX2
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: PGP1 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176364 Copy
Species: Homo sapiens
Genetic Insert: OSR1
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: SK-N-SH cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176363 Copy
Species: Homo sapiens
Genetic Insert: Z3CH10
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: SK-N-SH cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176368 Copy
Species: Homo sapiens
Genetic Insert: POU5F1
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: WTC11 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176367 Copy
Species: Homo sapiens
Genetic Insert: NR2E3
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: A549 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176369 Copy
Species: Homo sapiens
Genetic Insert: ZNF608
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: Cloning source: PGP1 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176362 Copy
Species: Atlantic deep subsurface metagenome
Genetic Insert: human codon optimized Cas13bt1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176316 Copy
Species: Synthetic
Genetic Insert: MCS-P2A-EGFP
Vector Backbone Description: Backbone Marker:Vector Builder; Backbone Size:4643; Vector Backbone:pAAV-SynI; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176276 Copy
Species: Mus musculus
Genetic Insert: Kir2.1-P2A-EGFP
Vector Backbone Description: Backbone Marker:Vector Builder; Backbone Size:4638; Vector Backbone:pAAV-SynI; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: Several differences were found flanking/near the lox and frt sites. These base changes do not affect the coding sequence or recombination sites.
Proper citation: RRID:Addgene_176278 Copy
Species:
Genetic Insert: tetR
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:pCDH; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176312 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ub CPS (8-16)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:parallel GST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The vector backbone, parallel GST2, was made by replacing the polylinker of pGEX4T1 with the polylinker isolated from the baculovirus expression plasmid pFastBac (see PMID: 10024467).
Proper citation: RRID:Addgene_17631 Copy
Species:
Genetic Insert: Cas13bt1 crRNA direct repeat
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176314 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Osh4
Vector Backbone Description: Backbone Size:5000; Vector Backbone:modified pGEX-4T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: DNA encoding Osh4 residues 2–434 was amplified by PCR and subcloned into the BamHI and XhoI sites of a pGEX-4T vector modified to contain a TEV protease recognition site.
Proper citation: RRID:Addgene_17632 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Vps23(1-385) – His/Vps28(1-242) – Vps37(1-213) – Mvb12(1-101)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pST39; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: DNAs for Mvb12, Vps37, Vps28, and Vps23 were cloned into pST39.
Proper citation: RRID:Addgene_17634 Copy
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