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Species: humanized S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9
Vector Backbone Description: Backbone Marker:Kuehn lab; Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene 64324), modification of pX330 (Addgene 42230); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31597252
Proper citation: RRID:Addgene_135013 Copy
Species: humanized S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9
Vector Backbone Description: Backbone Marker:Kuehn lab; Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene 64324), modification of pX330 (Addgene 42230); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31597252
Proper citation: RRID:Addgene_135014 Copy
Species: Synthetic
Genetic Insert: mCh-Nluc-P2A-neo
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:2300; Vector Backbone:pUC19; Vector Types:Cryptosporidium Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31231046
Proper citation: RRID:Addgene_135015 Copy
Species: Rattus norvegicus
Genetic Insert: Myo 9a (rat) with 5' UTR. GAP minus mutant.
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4719; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9819351
Comments: Please note: Plasmid contains a V2022I mutation in Myo 9a-GFP. This mutation is not a concern for plasmid function.
Proper citation: RRID:Addgene_134962 Copy
Species: Lamprey
Genetic Insert: LjCDA1L1_4
Vector Backbone Description: Vector Backbone:Px330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31399578
Proper citation: RRID:Addgene_135019 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9-P2A-mNeonGreen
Vector Backbone Description: Backbone Size:7926; Vector Backbone:L40C; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31735627
Comments: pL40C_PGKintron_Cas9_Green was derived by subcloning the hPGK promoter with beta-globin intron from Tet-pLKO-puro (Addgene #21915) into the L40C-CRISPR.EFS.mNeon.NL.SL vector (a gift from Dirk Heckl, Martin Luther University Halle-Wittenberg) using PacI and BamHI sites.
Proper citation: RRID:Addgene_134966 Copy
Vector Backbone Description: Backbone Size:7076; Vector Backbone:pLentiCRISPR-tagBFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31735627
Comments: The mini Fgf4 promoter is from FpG5 (Addgene #69443).
Proper citation: RRID:Addgene_134967 Copy
Species: Lamprey
Genetic Insert: LpCDA1L1_1
Vector Backbone Description: Vector Backbone:Px330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31399578
Proper citation: RRID:Addgene_135023 Copy
Species: Caenorhabditis elegans
Genetic Insert: ced-4
Vector Backbone Description: Vector Backbone:pXHA; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688797
Proper citation: RRID:Addgene_134970 Copy
Vector Backbone Description: Backbone Size:4723; Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134979 Copy
Species: Lamprey
Genetic Insert: LjDA1L2_2
Vector Backbone Description: Vector Backbone:Px330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31399578
Proper citation: RRID:Addgene_135034 Copy
Species: Aequorea victoria
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:8572; Vector Backbone:pCCL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32887975
Proper citation: RRID:Addgene_134984 Copy
Species: Entacmaea quadricolor
Genetic Insert: TagBFP
Vector Backbone Description: Backbone Size:8572; Vector Backbone:pCCL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32887975
Proper citation: RRID:Addgene_134986 Copy
Species: Homo sapiens
Genetic Insert: Myo 19
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4713; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30111583
Comments: this article is published:
Stefanie J. Oeding, Katarzyna Majstrowicz, Xiao-Ping Hu, Vera Schwarz, Angelika Freitag, Ulrike Honnert, Petra Nikolaus, Martin Bähler
Journal of Cell Science 2018 131: jcs219469 doi: 10.1242/jcs.219469 Published 10 September 2018
The Myo 19 cDNA was obtained from NITE Biological Resource Center (NBRC), Japan.
cDNA clone AK304073/FLJ61052/TRACH3006685
Proper citation: RRID:Addgene_134988 Copy
Species: Rattus norvegicus
Genetic Insert: Myo 1e (rat) with 3' UTR
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4696; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:7730414
Proper citation: RRID:Addgene_134905 Copy
Species: Homo sapiens
Genetic Insert: SMURF1
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10458166
Comments: Vector has TEV site.
A human Smurf1 cDNA encoding all but the first eight amino acids was identified in the expressed-sequence-tag database (AA292123), and full-length hSmurf1 was reconstituted using a fragment of XSmurf1 cDNA encoding the first eight amino acids.
Proper citation: RRID:Addgene_13503 Copy
Species: Homo sapiens
Genetic Insert: SMURF2
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11163210
Proper citation: RRID:Addgene_13504 Copy
Species: Mus musculus
Genetic Insert: Myo 1g
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4701; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19968988
Proper citation: RRID:Addgene_134991 Copy
Species: Synthetic
Genetic Insert: pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
Vector Backbone Description: Backbone Marker:Keith Joung Lab; Vector Backbone:MSP2638; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30528434
Proper citation: RRID:Addgene_134996 Copy
Species: Rattus norvegicus
Genetic Insert: Myo 9b (rat) with 3' UTR. GAP minus mutant R1695M
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4686; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9348541
Comments: See also:
Van den Boom et al.
Molecular Biology of the Cell Vol. 18, No. 4, 1507-1518, April 2007
The Myosin IXb Motor Activity Targets the Myosin IXb RhoGAP Domain as Cargo to Sites of Actin Polymerization.
Published Online:21 Feb 2007https://doi.org/10.1091/mbc.e06-08-0771
Please note: Plasmid contains a T721I mutation in Myo9b. This mutation is not known to affect protein function.
Proper citation: RRID:Addgene_134911 Copy
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