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Species: Homo sapiens
Genetic Insert: MEK1 (1-201)-MEK5 (498–1344) fusion
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24717435
Comments: The sequence discrepancies found during the QC process have no functional consequence.
Proper citation: RRID:Addgene_53176 Copy
Species: Homo sapiens
Genetic Insert: MEK1 (1-201)-MEK5 (498–1344) fusion
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZLblasti; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24717435
Comments: The sequence discrepancies found during the QC process have no functional consequence.
Proper citation: RRID:Addgene_53177 Copy
Species: Homo sapiens
Genetic Insert: ERK2
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZLblasti; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24717435
Comments: The sequence discrepancies found during the QC process have no functional consequence.
Proper citation: RRID:Addgene_53174 Copy
Species: Other
Genetic Insert: kanMX6
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17942599
Comments: This plasmid is similar to Addgene plasmid #39292, except the GFP has been switched to a more stable variant.
Proper citation: RRID:Addgene_53183 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TRP1
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17942599
Comments: This plasmid is similar to Addgene plasmid #41597, except the GFP has been switched to a more stable variant.
Proper citation: RRID:Addgene_53184 Copy
Genetic Insert: pCSaE500
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pCDFDuet-1; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23169002
Proper citation: RRID:Addgene_53182 Copy
Species: Homo sapiens
Genetic Insert: MEK1
Vector Backbone Description: Backbone Marker:Rinehart lab, Addgene plasmid 52053; Vector Backbone:pCRT7; Vector Types:Bacterial Expression, phosphoprotein synthesis; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_53225 Copy
Vector Backbone Description: Backbone Size:3504; Vector Backbone:pzDonor; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25122746
Proper citation: RRID:Addgene_53189 Copy
Vector Backbone Description: Backbone Size:3515; Vector Backbone:pzDonor; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25122746
Proper citation: RRID:Addgene_53188 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIs3MX6
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17942599
Comments: This plasmid is similar to Addgene plasmid #41598, except the GFP has been switched to a more stable variant.
Proper citation: RRID:Addgene_53185 Copy
Species: Homo sapiens
Genetic Insert: Par3
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_53229 Copy
Species: Other
Genetic Insert: URA3-KanMX6
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23878277
Proper citation: RRID:Addgene_53194 Copy
Species: Homo sapiens
Genetic Insert: MEK1
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24717435
Proper citation: RRID:Addgene_53195 Copy
Species: Rattus norvegicus
Genetic Insert: Syntaxin 1A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23536066
Proper citation: RRID:Addgene_53236 Copy
Species: Rattus norvegicus
Genetic Insert: VAMP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23536066
Proper citation: RRID:Addgene_53233 Copy
Species: Synthetic
Genetic Insert: monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043132
Proper citation: RRID:Addgene_53234 Copy
Species: Homo sapiens
Genetic Insert: Par4
Vector Backbone Description: Backbone Marker:Mark Davis lab; Vector Backbone:pBJ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_53231 Copy
Species: Homo sapiens
Genetic Insert: MEK5
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24717435
Comments: The sequence discrepancies found during the QC process have no functional consequence.
Proper citation: RRID:Addgene_53199 Copy
Species: Homo sapiens
Genetic Insert: MEK1
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24717435
Comments: The N12D mutation found during the QC process has no functional consequence.
Proper citation: RRID:Addgene_53196 Copy
Species: Homo sapiens
Genetic Insert: MEK1
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24717435
Comments: The S241P mutation found during the QC process has no functional consequence.
This plasmid is prone to recombination. We recommend screening 2-3 colonies to ensure that the full plasmid is selected.
Proper citation: RRID:Addgene_53197 Copy
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