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Species: Synthetic
Genetic Insert: W-TAGA qtRNA
Vector Backbone Description: Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34588441
Comments: The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI.
Proper citation: RRID:Addgene_134805 Copy
Species: Synthetic
Genetic Insert: Y-TAGA-Evo1 qtRNA
Vector Backbone Description: Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34588441
Comments: The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI.
Proper citation: RRID:Addgene_134808 Copy
Vector Backbone Description: Backbone Size:6742; Vector Backbone:pPZP200; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: Contains the 564bp bialaphos resistance (bar) gene from Streptomyces hygroscopicus
Proper citation: RRID:Addgene_134770 Copy
Vector Backbone Description: Backbone Size:6742; Vector Backbone:pPZP200; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16249869
Comments: Contains the 564bp bialaphos resistance (bar) gene from Streptomyces hygroscopicus
Proper citation: RRID:Addgene_134771 Copy
Species: Synthetic
Genetic Insert: SponGee-Kras
Vector Backbone Description: Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31242429
Proper citation: RRID:Addgene_134777 Copy
Species: Synthetic
Genetic Insert: ThPDE5VV
Vector Backbone Description: Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31242429
Proper citation: RRID:Addgene_134778 Copy
Species: Homo sapiens
Genetic Insert: METTL3/METTL14 methyltransferase domain and inactive dCas9
Vector Backbone Description: Backbone Size:3699; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31383972
Proper citation: RRID:Addgene_134779 Copy
Genetic Insert: pIII-29-TAGA
Vector Backbone Description: Backbone Size:3017; Vector Backbone:KanR. SC101 origin with RepA-E93K mutation; copy # ~27 copies/cell.; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34588441
Proper citation: RRID:Addgene_134812 Copy
Genetic Insert: pIII 16-TAGA 29-TAGA 34-TAGA
Vector Backbone Description: Backbone Size:3017; Vector Backbone:KanR. SC101 origin with RepA-E93K mutation; copy # ~27 copies/cell.; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34588441
Proper citation: RRID:Addgene_134813 Copy
Species: Escherichia coli
Genetic Insert: Dihydrofolate reductase
Vector Backbone Description: Backbone Marker:Novagen (EMD Millipore); Backbone Size:4008; Vector Backbone:pACYC-Duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32701056
Comments: Derived from Addgene plasmid 91226.
Proper citation: RRID:Addgene_134815 Copy
Species: Escherichia coli
Genetic Insert: Dihydrofolate reductase
Vector Backbone Description: Backbone Marker:Novagen (EMD Millipore); Backbone Size:4008; Vector Backbone:pACYC-Duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32701056
Proper citation: RRID:Addgene_134817 Copy
Species: Escherichia coli
Genetic Insert: Dihydrofolate reductase
Vector Backbone Description: Backbone Marker:Novagen (EMD Millipore); Backbone Size:4008; Vector Backbone:pACYC-Duet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32701056
Proper citation: RRID:Addgene_134818 Copy
Species: Mus musculus
Genetic Insert: Fstl1
Vector Backbone Description: Backbone Marker:See Rupp et al (1994) Genes Dev 8:1311; Backbone Size:5747; Vector Backbone:pCS2+luc; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17030984
Comments: See author's map for picture of empty pCS2-luc.
Proper citation: RRID:Addgene_13498 Copy
Species: Homo sapiens
Genetic Insert: MyoD1-Estrogen Receptor Fusion Protein
Vector Backbone Description: Backbone Marker:Morgenstern&Land (NAR 18:3587); Available at Addgene; Backbone Size:5100; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17030984
Comments: The Entrez ID for MyoD is NM_010866 ; the Entrez ID for ER is NM_000125. See pBABE MDER for map of wild-type plasmid.
Proper citation: RRID:Addgene_13495 Copy
Species: Synthetic
Genetic Insert: pUBC-DpnI(aa146-254)-3XFLAG-Dam(R95A) pGK-HygroR
Vector Backbone Description: Backbone Marker:David Baltimore Lab Addgene #14883; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30528434
Proper citation: RRID:Addgene_135001 Copy
Species: Nostoc sp. PCC 7906
Genetic Insert: MusF2 prenyltransferaseM
Vector Backbone Description: Vector Backbone:pRSF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31674754
Comments: It was published as Addgene ID # 135005
Proper citation: RRID:Addgene_135005 Copy
Species: Nostoc sp. UHCC 0398
Genetic Insert: MusF1 prenyltransferase
Vector Backbone Description: Vector Backbone:pRSF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31674754
Comments: It was published as Addgene ID # 135006
Proper citation: RRID:Addgene_135006 Copy
Species: Rattus norvegicus
Genetic Insert: Myo 9a (rat) with 5' UTR
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4719; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9819351
Proper citation: RRID:Addgene_134959 Copy
Species: humanized S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid # 42230); Backbone Size:8506; Vector Backbone:pX330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31597252
Proper citation: RRID:Addgene_135011 Copy
Species: humanized S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9
Vector Backbone Description: Backbone Marker:Kuehn lab; Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene 64324), modification of pX330 (Addgene 42230); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31597252
Proper citation: RRID:Addgene_135012 Copy
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