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Species: Hepatitis B virus
Genetic Insert: HBSP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6360; Vector Backbone:pBIND; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53113 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:JBEI; Backbone Size:8000; Vector Backbone:pBullet; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: See http://gt.jbei.org/ for more information on the JBEI glycosyltransferase collection.
Proper citation: RRID:Addgene_53078 Copy
Species: Drosophila melanogaster
Genetic Insert: 5HT7
Vector Backbone Description: Vector Backbone:pME18ST; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments: Note that this plasmid has a few differences from the Entrez Genbank reference sequence for Drosophila 5-HT7. It was cloned from a w1118 fly strain and can be considered a functional variant.
Plasmid can be directly used for expression in HEK cells. Insert cloned into the NotI and XhoI sites. Has a vertebrate Kozak sequence (GCCACC) immediately upstream of the ATG.
Proper citation: RRID:Addgene_53111 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:JBEI; Backbone Size:8000; Vector Backbone:pBullet; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: See http://gt.jbei.org/ for more information on the JBEI glycosyltransferase collection.
Proper citation: RRID:Addgene_53076 Copy
Species: Homo sapiens
Genetic Insert: OCT1
Vector Backbone Description: Vector Backbone:pLenti-CMV-BSD; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53118 Copy
Species: Staphylococcus aureus
Genetic Insert: theophylline riboswitch and alpha hemolysin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5442; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53116 Copy
Species: Thermus thermophilus
Genetic Insert: TtAgo
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:3621; Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_53082 Copy
Species: Homo sapiens
Genetic Insert: Gfa2
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
References:
Comments: The plasmid is a pUC18 vector containing the human GFAP sequences from -2163 to +47 (the human gfa2 segment), with the initiating ATG mutated to TTG, placed in front of the nuclear targeted E. coli lacZ gene, which in turn is followed by a fragment of the mouse protamine-1 gene. The latter supplies an intron, stabilizing 3' UTR, and a polyadenylation signal. If cloning a cDNA, it is advisable to retain the mP-1 segment. The lacZ gene can be excised by digestion with BamHI, and replaced with your gene of interest. If cloning a genomic sequence that includes an intron and polyadenylation site (this may compromise astrocyte specificity--see Su et al. 2004), the mP-1 region can also be excised, or alternatively, a number of sites flank the gfa2 segment allowing its isolation.
Restriction sites that may be useful are as follows:
EcoRI: flanks the gfa2, lacZ, and mP-1 segments
Bgl II: 5' flank of gfa2 & 3' flank of mP-1
Bam HI: flanks the lacZ gene
Sal I: cuts uniquely between the gfa2 promoter and the LacZ gene
Sph I and Hind III: cut uniquely just 3' of the mP-1 segment
Although the GFAP promoter appears to be the best choice for targeting transgene expression to astrocytes in mice, please be aware that neuronal expression has also occasionally been observed (Su et al. 2004).
Proper citation: RRID:Addgene_53126 Copy
Species: Arabidopsis thaliana
Genetic Insert: zep
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:10040; Vector Backbone:pAC-ZEAXipi; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
References:
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53087 Copy
Species: Aphelenchus avenae
Genetic Insert: AavLEA1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5708; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53093 Copy
Species: E. coli
Genetic Insert: fimE
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53015 Copy
Species: Mus musculus
Genetic Insert: Ubc9
Vector Backbone Description: Backbone Size:3666; Vector Backbone:pET23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53137 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53013 Copy
Species: E.coli
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53014 Copy
Species: E.coli
Genetic Insert: DsrA sRNA
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53011 Copy
Species: Homo sapiens
Genetic Insert: Gfa2
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid is a variant of pGfa2-nLac (Addgene plasmid #53126) constructed by inserting 3 copies of the gfa2 B segment (Besnard et al. 1991) into the Sma I site just upstream of the basal promoter. It has about 75 times higher activity than pGfa2-nLac in transfected cells, but does not work in transgenic mice; possibly the high level of expression it produces is toxic.
Proper citation: RRID:Addgene_53132 Copy
Species: Aequorea victoria
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53017 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53018 Copy
Species: E.coli
Genetic Insert: csrA
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_53026 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_53024 Copy
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