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Genetic Insert: lysogen of a temperature-inducible bacteriophage lambda
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:28522818
Comments: Genotype is [λ+ Lac- galK2 IN(rrnD-rrnE)1 rph-1], a derivative of strain W3102
Phenotypic assay: Grow at 30°C, but restricted at 42°C due to the induction of phage particles and cell lysis.
Proper citation: RRID:Addgene_134836 Copy
Species: Homo sapiens
Genetic Insert: hGADD45A-PUFa-hTET1CD(1418-2136)
Vector Backbone Description: Backbone Marker:Plasmid #25896; Backbone Size:9573; Vector Backbone:pLX302; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134837 Copy
Species: Mus musculus
Genetic Insert: MKP-1ASA
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pWay21 EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15899879
Proper citation: RRID:Addgene_13479 Copy
Species: Mus musculus
Genetic Insert: MKP-1L17A
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pWay21 EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15899879
Proper citation: RRID:Addgene_13477 Copy
Species: Homo sapiens
Genetic Insert: Flag-NEILE2-PUFa-TET1(CD)
Vector Backbone Description: Backbone Marker:Plasmid #25896; Backbone Size:9573; Vector Backbone:pLX302; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134849 Copy
Species: Homo sapiens
Genetic Insert: HRas 61L
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8668210
Comments: H-ras 61L is GTPase deficient, constitutively activated. This plasmid contains 600 bp H-ras and 500 bp 3' untranslated region.
Proper citation: RRID:Addgene_13485 Copy
Species: Homo sapiens
Genetic Insert: PUFa-NEIL2-TET1(CD)
Vector Backbone Description: Backbone Marker:Plasmid #25896; Backbone Size:9573; Vector Backbone:pLX302; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134850 Copy
Species: Homo sapiens
Genetic Insert: PUFc-NEIL2
Vector Backbone Description: Backbone Marker:Plasmid #25896; Backbone Size:9573; Vector Backbone:pLX302; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134851 Copy
Species: Homo sapiens
Genetic Insert: Ras-related protein 3C
Vector Backbone Description: Backbone Size:8825; Vector Backbone:pLVX-EF1a-IRES-Puromycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31968357
Proper citation: RRID:Addgene_134857 Copy
Species: Homo sapiens
Genetic Insert: Ras-related protein 5A
Vector Backbone Description: Backbone Size:8825; Vector Backbone:pLVX-EF1a-IRES-Puromycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31968357
Proper citation: RRID:Addgene_134858 Copy
Species: Mus musculus
Genetic Insert: ERGIC53
Vector Backbone Description: Backbone Size:8825; Vector Backbone:pLVX-EF1a-IRES-Puromycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31968357
Proper citation: RRID:Addgene_134859 Copy
Species: Homo sapiens
Genetic Insert: Membrin
Vector Backbone Description: Backbone Size:8825; Vector Backbone:pLVX-EF1a-IRES-Puromycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31968357
Proper citation: RRID:Addgene_134865 Copy
Species: Homo sapiens
Genetic Insert: cytochrome b5 type A
Vector Backbone Description: Backbone Size:8825; Vector Backbone:pLVX-EF1a-IRES-Puromycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31968357
Proper citation: RRID:Addgene_134869 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-GADD45A-PUFa-dTET1(CD)H1671Y D1673A)
Vector Backbone Description: Backbone Marker:Plasmid #25896; Backbone Size:9573; Vector Backbone:pLX302; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134870 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-GADD45A(G39A)-PUFa-TET1(CD)
Vector Backbone Description: Backbone Marker:Plasmid #25896; Backbone Size:9573; Vector Backbone:pLX302; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134871 Copy
Species: Homo sapiens
Genetic Insert: 3xFlag-GADD45A(G39A L77E)-PUFa-TET1(CD)
Vector Backbone Description: Backbone Marker:Plasmid #25896; Backbone Size:9573; Vector Backbone:pLX302; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134873 Copy
Species: Homo sapiens
Genetic Insert: human MSH2_G674A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4776; Vector Backbone:pFastBac 1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21329650
Proper citation: RRID:Addgene_134874 Copy
Species: Synthetic
Genetic Insert: CRISPR/Cas9
Vector Backbone Description: Vector Backbone:pGreen3; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31558579
Proper citation: RRID:Addgene_134757 Copy
Species: Synthetic
Genetic Insert: Wildtype S tRNA (decodes TCG)
Vector Backbone Description: Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34588441
Comments: The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI.
Proper citation: RRID:Addgene_134803 Copy
Species: Synthetic
Genetic Insert: Wildtype S tRNA (decodes TCG)
Vector Backbone Description: Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34588441
Comments: The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI.
Proper citation: RRID:Addgene_134804 Copy
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