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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pBx1-VHH template2-3XMyc-Spacer
 
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RRID:Addgene_176209 VHH template2-3XMyc-Spacer Synthetic Ampicillin PMID:34535642 Backbone Size:2561; Vector Backbone:pBx1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:08:54 0
pMC8b-XI1up
 
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RRID:Addgene_176166 5' homology for integration site XI-1 Saccharomyces cerevisiae Chloramphenicol PMID:34860007 Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-01 01:08:53 0
CAG.mCherry-eDHFR
 
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RRID:Addgene_176201 mCherry-eDHFR E. coli Ampicillin PMID:33904910 Please note that there are minor differences between the Addgene NGS result and the depositor's sequence. These differences do not alter plasmid function. Backbone Marker:Dr. Eugene Makeyev; Backbone Size:-1; Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:08:54 0
pMC7-XI1dw
 
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RRID:Addgene_176167 3' homology for integration site XI-1 Saccharomyces cerevisiae Chloramphenicol PMID:34860007 Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-01 01:08:53 0
pBH31(gfcD-10xHis)
 
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RRID:Addgene_176200 gfcD E. coli E2348/69 O127:H6 Ampicillin PMID:34780538 encodes (pelC signal sequence)-His10-GfcD(22-698) Backbone Marker:from Dr Frank Collart; Vector Backbone:pBH31; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:08:54 0
pMC7-XI2dw
 
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RRID:Addgene_176169 3' homology for integration site XI-2 Saccharomyces cerevisiae Chloramphenicol PMID:34860007 Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016). Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-01 01:08:54 0
CAG.mCherry-INBox-eDHFR
 
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RRID:Addgene_176202 mCherry-INBox-eDHFR Homo sapiens Ampicillin PMID:33904910 Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:08:54 0
EF.delmFasL.CMV.GFP
 
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1+ mentions
RRID:Addgene_17620 delFasL Mus musculus Ampicillin PMID:12788657 The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP, with a unique EcoRV site available to insert a gene of interest under the control of the EF promoter. Noncleavable mouse delFasL cDNA was constructed using PCR cloning to delete the exon 2 region, which contains the metalloproteinase cleavage site. The delFasL gene was then cloned into the EcoRV site of EF.CMV.GFP to make the EF.delFasL-CMV.GFP LV. Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes. Backbone Size:0; Vector Backbone:EF.CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Noncleavable mouse delFasL . Deleted the exon 2 region, which contains the metalloproteinase cleavage site. 2026-08-01 01:08:54 1
CAG.MCAK-mCherry-eDHFR
 
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RRID:Addgene_176204 MCAK-mCherry-eDHFR Homo sapiens Ampicillin PMID:33904910 Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:08:54 0
EF.hICN1.CMV.GFP
 
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10+ mentions
RRID:Addgene_17623 ICN1 Homo sapiens Ampicillin PMID:16513761 Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function. The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP. hICN1 was cloned downstream of the EF-1a promoter. Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes. Backbone Marker:Linzhao Cheng Lab; Backbone Size:8200; Vector Backbone:EF.v-CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:08:54 17
EF.hICN1.Ubc.GFP
 
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1+ mentions
RRID:Addgene_17626 ICN1 Homo sapiens Ampicillin PMID:16513761 Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function. hICN1 driven by the EF-1a promoter and a GFP reporter driven by the ubiquitin C (Ubc) promoter. Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes. Backbone Size:11500; Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:08:55 8
Duet011
 
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1+ mentions
RRID:Addgene_17627 UBC promoter - GFP - PGK promoter - Hygro Ampicillin PMID:17158240 Lentiviral dual promoter/dual-gene expression vector is dubbed Duet (for Dual-gene, excisable transgene). Backbone Size:0; Vector Backbone:Duet011; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:08:55 4
pNOC_hfnCas12a-Nlux
 
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RRID:Addgene_176239 humanized fnCas12a Francisella novicida Ampicillin PMID:34793143 Vector Backbone:pUC19; Vector Types:CRISPR, Luciferase, Synthetic Biology, Expression in microalgae Nannochloropsis oceanica; Bacterial Resistance:Ampicillin 2026-08-01 01:08:54 0
pBH31(gfcD)
 
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RRID:Addgene_176199 gfcD E. coli E2348/69 O127:H6 Ampicillin PMID:34780538 encodes (pelC signal sequence)-His6-GfcD(22-698) Backbone Marker:from Dr Frank Collart; Vector Backbone:pBH31; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:08:54 0
pspCas9-POLI-ST2-com vector
 
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RRID:Addgene_176234 Ampicillin PMID:35323942 Vector Backbone:Addgene# 136269; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:08:54 0
pspCas9-3exo-Tetra-com-CLCN5-sp-g1
 
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RRID:Addgene_176236 spCas9 and polymerase exo domain fusion Homo sapiens Ampicillin PMID:35323942 Vector Backbone:pspCas9-3'UTR-Tetra-COM-Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:08:54 0
pspCas9-5Exo-tetra-com-hCLCN5-sp-g1
 
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RRID:Addgene_176237 spCas9 and polymerase exo domain fusion Homo sapiens Ampicillin PMID:35323942 Vector Backbone:pspCas9-3'UTR-Tetra-COM-Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:08:54 0
pSA10(gfcBCD)
 
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RRID:Addgene_176193 gfcBCD E. coli E2348/69 O127:H6 Ampicillin PMID:34780538 Insert is a contiguous set of genes gfcB, gfcC, gfcD. All 3 proteins are expressed with their native signal sequences Backbone Marker:from Dr Shoshi Altuvia; Vector Backbone:pSA10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:08:54 0
pSA10(gfcB-His-CD)
 
Resource Report
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RRID:Addgene_176194 gfcBCD E. coli E2348/69 O127:H6 Ampicillin PMID:34780538 Insert is a contiguous set of genes gfcB, gfcC, gfcD. All 3 proteins are expressed with their native signal sequences. Derived from plasmid pSA10(gfcBCD) [Addgene #176193] by mutagenesis to include a 6xHis tag on the C-term of GfcB. Backbone Marker:from Dr Shoshi Altuvia; Vector Backbone:pSA10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:08:54 0
pMCSG7(gfcC)
 
Resource Report
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RRID:Addgene_176197 gfcC E. coli E2348/69 O127:H6 Ampicillin PMID:34780538 encodes His6-(x)8-TeV-GfcC(17-214) Vector Backbone:pMCSG7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:08:54 0

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