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Species: Homo sapiens
Genetic Insert: E6-AP
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: In addition to C833A, there is also an L837V amino acid change. E6-AP is missing the first 22 amino acids of isoform 1 (protein starts with MEACT NEFCA).
Proper citation: RRID:Addgene_13465 Copy
Species: Synthetic
Genetic Insert: CRISPR/Cas9
Vector Backbone Description: Vector Backbone:pGreen3; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31558579
Proper citation: RRID:Addgene_134754 Copy
Species: Homo sapiens
Genetic Insert: UFM1 sgRNA2
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31595041
Proper citation: RRID:Addgene_134634 Copy
Species: Homo sapiens
Genetic Insert: UFL1 sgRNA1
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31595041
Proper citation: RRID:Addgene_134635 Copy
Species: Homo sapiens
Genetic Insert: UFL1 sgRNA2
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31595041
Proper citation: RRID:Addgene_134636 Copy
Species: Homo sapiens
Genetic Insert: UFSP2 sgRNA2
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31595041
Proper citation: RRID:Addgene_134637 Copy
Species: Homo sapiens
Genetic Insert: UFM1
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31595041
Proper citation: RRID:Addgene_134639 Copy
Species: Homo sapiens
Genetic Insert: UFM1
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31595041
Proper citation: RRID:Addgene_134640 Copy
Species: Homo sapiens
Genetic Insert: RPL26 sgRNA2
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31595041
Proper citation: RRID:Addgene_134641 Copy
Species: Homo sapiens
Genetic Insert: RPL26 sgRNA2
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31595041
Proper citation: RRID:Addgene_134642 Copy
Vector Backbone Description: Backbone Marker:Addgene 119262; Backbone Size:10856; Vector Backbone:pJMP1237; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31481541
Comments: The parent plasmid was used in this paper: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424567/#SD7
We obtained the new promoter from the Biobrick registry.
Proper citation: RRID:Addgene_134657 Copy
Species: Homo sapiens
Genetic Insert: METTL3/METTL14 methyltransferase domain and inactive dCas9
Vector Backbone Description: Backbone Size:3699; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31383972
Proper citation: RRID:Addgene_134780 Copy
Species: Homo sapiens
Genetic Insert: FTO and inactive dCas9
Vector Backbone Description: Backbone Size:3421; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31383972
Proper citation: RRID:Addgene_134781 Copy
Species: Homo sapiens
Genetic Insert: FTO and inactive dCas9
Vector Backbone Description: Backbone Size:3421; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31383972
Proper citation: RRID:Addgene_134782 Copy
Species: Homo sapiens
Genetic Insert: ALKBH5 and inactive dCas9
Vector Backbone Description: Backbone Size:3421; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31383972
Proper citation: RRID:Addgene_134784 Copy
Species: Homo sapiens
Genetic Insert: Phosphoserine phosphatase
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:7377; Vector Backbone:pCDH-CMV-MCS-EF1-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31446747
Proper citation: RRID:Addgene_134786 Copy
Genetic Insert: luxAB-357-CGGC
Vector Backbone Description: Backbone Size:3330; Vector Backbone:KanR SC101; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34588441
Comments: Genes luxCDE are also required to generate the luciferase substrate. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode luxCDE.
Proper citation: RRID:Addgene_134793 Copy
Species: Synthetic
Genetic Insert: R-TAGA-Evo1 qtRNA
Vector Backbone Description: Vector Backbone:RK2 SpecR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34588441
Comments: The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI.
Proper citation: RRID:Addgene_134798 Copy
Species: Rattus norvegicus
Genetic Insert: Myo 1c (rat) with 3' UTR
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4708; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8719884
Proper citation: RRID:Addgene_134832 Copy
Genetic Insert: 24xGCN4_v4-2xIFP2-CAAX
Vector Backbone Description: Vector Backbone:pHRdSV40-UP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27153498
Comments: Please note: Plasmid contains a N7D mutation in the 23rd GCN4_v4 tag. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_134833 Copy
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