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Species: Mus musculus
Genetic Insert: Sox2
Vector Backbone Description: Backbone Size:6800; Vector Backbone:pCAG-HA-gw-IP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15863505
Comments: The Sox2 cDNA contains a modified ATG start codon to TTG for cloning into vectors with an N-terminal tag.
Proper citation: RRID:Addgene_13459 Copy
Species: Mus musculus
Genetic Insert: Cyp26a1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5-HS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16818722
Comments: Full-length cDNA for mouse Cyp26a1 cloned into modified pSG5. See Author's map for more information.
Proper citation: RRID:Addgene_13457 Copy
Species: Homo sapiens
Genetic Insert: M1 Spastin
Vector Backbone Description: Vector Backbone:mApple-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31227594
Proper citation: RRID:Addgene_134461 Copy
Vector Backbone Description: Backbone Size:5528; Vector Backbone:pDW1775; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17406455
Comments: Plant zinc finger nuclease cloning vector. Zinc fingers are cloned in between unique XbaI and BamHI sites in front of the FokI nuclease domain.
pDW1775 consists of the following components in the given order: Hinc II, SexA I, EcoN I, lambda T0 terminator, T7 primer site, BsiW I, rrnB terminator, modified NOS promoter, Bgl II, Xho I, plant leader, MASS translation signal, SV40 nuclear localization signal, AcV5 epitope tag, Xba I, Xma I, Age I, BamH I, plant optimized Fok I nuclease domain, Sac I, NOS terminator, Apa I, T3 primer site, phage FD gene VIII terminator, Bpu 10 I, modified yeast His3 gene, Pac I, yeast CEN/ARS, Bsu36 I (not unique), modified beta lactamase gene (ampicillin resistance), E. coli Trp A terminator, BstE II, Blp I, PpuM I, Rsr II, PflF I, Bgl I, modified high copy ColEI replicon, and back to Hinc II. This vector can be maintained in E. coli and yeast (Saccharomyces cerevisiae) and is also able to express a zinc finger nuclease in yeast and plants using the modified NOS promoter to drive expression. Additionally, this vector was designed to have minimal expression of the zinc finger nuclease in E. coli to facilitate cloning and reduce toxicity effects.
Proper citation: RRID:Addgene_13456 Copy
Species: Drosophila melanogaster
Genetic Insert: gRNAs for knockout of Drosophila genes
Vector Backbone Description: Vector Backbone:pLib6.4; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30051818
Proper citation: RRID:Addgene_134582 Copy
Species: Homo sapiens
Genetic Insert: M1 Spastin
Vector Backbone Description: Vector Backbone:mApple-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31227594
Proper citation: RRID:Addgene_134462 Copy
Species: Homo sapiens
Genetic Insert: M1 Spastin
Vector Backbone Description: Vector Backbone:mApple-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31227594
Proper citation: RRID:Addgene_134463 Copy
Species: Drosophila melanogaster
Genetic Insert: gRNAs for knockout of Drosophila genes
Vector Backbone Description: Vector Backbone:pLib6.4; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30051818
Proper citation: RRID:Addgene_134584 Copy
Species: Homo sapiens
Genetic Insert: M1 Spastin
Vector Backbone Description: Vector Backbone:mApple-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31227594
Proper citation: RRID:Addgene_134464 Copy
Species: Homo sapiens
Genetic Insert: M1 Spastin Amino Acids 1-92
Vector Backbone Description: Vector Backbone:mEmerald-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31227594
Proper citation: RRID:Addgene_134465 Copy
Species: Mus musculus
Genetic Insert: capping protein alpha 2 beta 2 (mouse)
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:5000; Vector Backbone:pet 24 a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16895918
Proper citation: RRID:Addgene_13452 Copy
Species: Homo sapiens
Genetic Insert: M1 Spastin Amino Acids 1-92
Vector Backbone Description: Vector Backbone:mApple-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31227594
Proper citation: RRID:Addgene_134466 Copy
Species: Mus musculus
Genetic Insert: KIF1Bbeta
Vector Backbone Description: Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31461551
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Addgene NGS identified the following amino acid discrepancies relative to Genbank accession: NP_997565.2: V406A, L536I, S542N, Y939C, Q1093R, T1187I, S1766N. The depositing laboratory has confirmed that the plasmid should function as described in their publication.
Proper citation: RRID:Addgene_134620 Copy
Species: Homo sapiens
Genetic Insert: M1 Spastin Amino Acids 1-92
Vector Backbone Description: Vector Backbone:DsRed2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31227594
Proper citation: RRID:Addgene_134467 Copy
Species: Mus musculus
Genetic Insert: capping protein alpha 1 beta 2 (mouse)
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:5000; Vector Backbone:pET- 3d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12956956
Proper citation: RRID:Addgene_13450 Copy
Species: Homo sapiens
Genetic Insert: DDX43-N-terminal (1-253 aa) with G46D mutation
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28468824
Proper citation: RRID:Addgene_134588 Copy
Species: Homo sapiens
Genetic Insert: KIF1C
Vector Backbone Description: Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31461551
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Proper citation: RRID:Addgene_134621 Copy
Species: Homo sapiens
Genetic Insert: GPAT4
Vector Backbone Description: Vector Backbone:mEmerald-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31227594
Proper citation: RRID:Addgene_134468 Copy
Species: Other
Genetic Insert: mdlC (benzylformate decarboxylase) C -terminal his tag
Vector Backbone Description: Backbone Marker:None; Backbone Size:2100; Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32441815
Comments: Please visit https://www.biorxiv.org/content/10.1101/820787v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_134590 Copy
Genetic Insert: Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
Vector Backbone Description: Vector Backbone:pCAG-CFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31570596
Proper citation: RRID:Addgene_134470 Copy
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