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Species: MS2 Phage
Genetic Insert: Maturation Protein
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2200; Vector Backbone:pMX; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_175953 Copy
Species: MS2 Phage
Genetic Insert: Maturation Protein
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2200; Vector Backbone:pMX; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_175954 Copy
Species: Danio rerio
Genetic Insert: Cavin4b
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176008 Copy
Species: Other
Genetic Insert: EGFP with nucleus-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_175839 Copy
Species: Mus musculus
Genetic Insert: Cavin4
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176007 Copy
Species: MS2 Phage
Genetic Insert: Maturation Protein
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2200; Vector Backbone:pMX; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_175952 Copy
Species: Homo sapiens
Genetic Insert: WIPI2B (S395E)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6143; Vector Backbone:pHTN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176006 Copy
Species: Homo sapiens
Genetic Insert: OPN-NT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5300; Vector Backbone:pDest490; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17594 Copy
Species: Homo sapiens
Genetic Insert: IFN gamma promoter with 1st intron enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Young lab stock #D3314.
SalI should give 8.6kb + 0.6kb bands.
Proper citation: RRID:Addgene_17598 Copy
Species: Homo sapiens
Genetic Insert: IFN gamma promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Young lab stock #D3299.
SalI should give 8.5kb band
Proper citation: RRID:Addgene_17599 Copy
Species: Homo sapiens
Genetic Insert: ZBED9-Nickase1
Vector Backbone Description: Backbone Marker:addgene ID: 62987; Vector Backbone:pX462; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: gRNA sequence also includes uncloned PAM
Proper citation: RRID:Addgene_175854 Copy
Species: Homo sapiens
Genetic Insert: CHCHD3-Nickase1
Vector Backbone Description: Backbone Marker:addgene ID: 62987; Vector Backbone:pX462; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: gRNA sequence also includes uncloned PAM
Proper citation: RRID:Addgene_175857 Copy
Species: Danio rerio
Genetic Insert: Bin1a
Vector Backbone Description: Vector Backbone:SITS-mCherry-ccdb (Addgene 118629); Vector Types:Leishmania cell free expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176024 Copy
Species:
Genetic Insert: rtTA3
Vector Backbone Description: Backbone Marker:System Biosciences; Vector Backbone:pCDH; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176027 Copy
Species: Homo sapiens
Genetic Insert: CDX4-Nickase1
Vector Backbone Description: Backbone Marker:addgene ID: 62987; Vector Backbone:pX462; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: gRNA sequence also includes uncloned PAM
Proper citation: RRID:Addgene_175852 Copy
Species: Danio rerio
Genetic Insert: Bin1a
Vector Backbone Description: Vector Backbone:SITS-EGFP-ccdb (Addgene 118628); Vector Types:Leishmania cell free expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_176020 Copy
Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17612 Copy
Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRK5-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17613 Copy
Species: Homo sapiens
Genetic Insert: EF-1a promoter
Vector Backbone Description: Backbone Marker:Dr. Didier Trono; Backbone Size:0; Vector Backbone:SIN LV pRLLhPGK.GFP Sin-18 (modified); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: SIN lentiviral vector that expresses GFP controlled by the promoter of the housekeeping gene EF-1alpha.
We constructed EF.GFP by replacing the human PGK promoter (EcoRV to BamHI sites in the PGK.GFP vector) with human EF-1alpha promoter from plasmid pEF1/Myc-His-A (Invitrogen, Carlsbad, CA). The EF-1alpha promoter (nucleotide 373 to 1560 as numbered in GenBank accession J04616) contains the first exon, first intron, and part of the second exon before the coding sequence (starting at nucleotide 1582).
Recombinant LVs were generated using the 3-plasmid system by cotransfection of 293T cells through calcium phosphate precipitation. The ratio of a transducing vector, pMD.G, and pCMVDeltaR8.91 was fixed at 1.5:0.5:2 µg for 10^6 293T cells.
Proper citation: RRID:Addgene_17616 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:MoClo assembly; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
References:
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015) from the part plasmids pYTK008, pYTK047, pYTK073, pYTK090, pMC6-spacer, pMC7-XII5dw, pMC8b-XII5up. Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176161 Copy
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