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Species: Other
Genetic Insert: tTA-ER
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; vector was modified by substituting mCherry for YFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20422051
Proper citation: RRID:Addgene_134567 Copy
Species: Other
Genetic Insert: tTA-ER
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20422051
Proper citation: RRID:Addgene_134568 Copy
Species: Other
Genetic Insert: tTA-ER
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; vector was modified by substituting CFP for YFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20422051
Proper citation: RRID:Addgene_134569 Copy
Species: Homo sapiens
Genetic Insert: IP3KC
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12747803
Proper citation: RRID:Addgene_134603 Copy
Species: Synthetic
Genetic Insert: D4H*
Vector Backbone Description: Backbone Size:5006; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31548609
Comments: https://benchling.com/s/seq-qYjuRHB1UTwPC8BVFbvK
Proper citation: RRID:Addgene_134604 Copy
Species: Homo sapiens
Genetic Insert: Flag-humanRBM47
Vector Backbone Description: Backbone Size:3960; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_134607 Copy
Vector Backbone Description: Backbone Marker:Feng Zhang; Vector Backbone:pSpCas9(BB)-2A-GFP (PX458); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32150173
Comments: The sgRNAs can get introduced by golden gate cloning like described in Andersson-Rolf A et al.: Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Dev Biol. 2016. doi: 10.1016/j.ydbio.2016.10.016. For more information see protocol.
Proper citation: RRID:Addgene_134450 Copy
Vector Backbone Description: Backbone Marker:Feng Zhang; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32150173
Comments: The sgRNAs can get introduced by golden gate cloning like described in Andersson-Rolf A et al.: Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Dev Biol. 2016. doi: 10.1016/j.ydbio.2016.10.016. For more information see protocol.
Proper citation: RRID:Addgene_134451 Copy
Species: Mus musculus
Genetic Insert: PCAF
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20422051
Proper citation: RRID:Addgene_134572 Copy
Species: Mus musculus
Genetic Insert: GCN5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20422051
Proper citation: RRID:Addgene_134573 Copy
Species: Homo sapiens
Genetic Insert: DDX43
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28468824
Proper citation: RRID:Addgene_134574 Copy
Genetic Insert: linker-mScarlet-I for C-terminal fusion
Vector Backbone Description: Vector Backbone:pMT178; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:31550057
Proper citation: RRID:Addgene_134455 Copy
Species: Synthetic
Genetic Insert: rbs-msfGFPC-H3H4
Vector Backbone Description: Vector Backbone:pHCL149; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31550057
Proper citation: RRID:Addgene_134456 Copy
Species: Synthetic
Genetic Insert: Para-popZ-rbs-H3H4-msfGFPN-CTM.
Vector Backbone Description: Vector Backbone:pTB285; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31550057
Proper citation: RRID:Addgene_134457 Copy
Vector Backbone Description: Backbone Marker:Feng Zhang; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32150173
Comments: The sgRNAs can get introduced by golden gate cloning like described in Andersson-Rolf A et al.: Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Dev Biol. 2016. doi: 10.1016/j.ydbio.2016.10.016. For more information see protocol.
Proper citation: RRID:Addgene_134458 Copy
Species: Mus musculus
Genetic Insert: KIF5B
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31461551
Proper citation: RRID:Addgene_134612 Copy
Species: Mus musculus
Genetic Insert: KIF3C
Vector Backbone Description: Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31461551
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Proper citation: RRID:Addgene_134616 Copy
Species: Homo sapiens
Genetic Insert: P10
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_1345 Copy
Species: Homo sapiens
Genetic Insert: CDK5
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_1346 Copy
Species: Mus musculus
Genetic Insert: KIF1Balpha
Vector Backbone Description: Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31461551
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
Proper citation: RRID:Addgene_134619 Copy
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