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Species: Homo sapiens
Genetic Insert: NRAS
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52728 Copy
Species: Homo sapiens
Genetic Insert: KRAS
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52729 Copy
Genetic Insert: KIlac strain
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:22605776
Comments: KIlac refers to the knock-in of the wild-type operon into the attTn7 locus; this strain was used as the reference strain for all analyses. We constructed all knock-in strains using the approach of McKenzie and Craig. The knock-in contained the entire lac operon starting 75bp upstream of lacI and ending 100bp downstream of lacA. The ΔZYA strain was transformed with pGRG37. The plasmid carried both the lac operon and the transposon genes tnsABCD, which allow for a site-specific insertion at attTn7.
Proper citation: RRID:Addgene_52696 Copy
Genetic Insert: OpLac1 strain
Vector Backbone Description: Vector Backbone:na; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:22605776
Comments: The sequence Oplac1 was synthesized using preferred codons at every position (GENEART), thus optimizing the codon adaptation index.
Proper citation: RRID:Addgene_52697 Copy
Species: Homo sapiens
Genetic Insert: HRAS
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24239284
Proper citation: RRID:Addgene_52730 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:2736; Vector Backbone:pX330; Vector Types:CRISPR, Toxoplasma gondii; Bacterial Resistance:Ampicillin
Comments: Cong L, Ran FA, Cox D, Lin S, Barretto R, et al. (2013) Multiplex genome engineering using CRISPR/Cas systems. Science 339: 819–823. doi:10.1126/science.1231143.
Proper citation: RRID:Addgene_52694 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: none
Vector Backbone Description: Vector Backbone:pREP41; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_52690 Copy
Species: Homo sapiens
Genetic Insert: Toll/IL-1 Receptor adaptor protein
Vector Backbone Description: Vector Backbone:MSCV2.2; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24529375
Proper citation: RRID:Addgene_52736 Copy
Species: Other
Genetic Insert: NGFP+Z-fusion
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5641; Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15631464
Proper citation: RRID:Addgene_52734 Copy
Genetic Insert: OpLac2 strain
Vector Backbone Description: Vector Backbone:na; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:22605776
Comments: Oplac2 was designed by a proprietary optimization strategy (developed by DNA 2.0) that incorporated codons for a subset of amino acids read by tRNAs that are the most highly charged during amino acid starvation.
Proper citation: RRID:Addgene_52699 Copy
Species: Other
Genetic Insert: link-NGFP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5641; Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15631464
Proper citation: RRID:Addgene_52732 Copy
Species: Homo sapiens
Genetic Insert: Toll/IL-1 Receptor adaptor protein
Vector Backbone Description: Vector Backbone:MSCV2.2; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24529375
Proper citation: RRID:Addgene_52739 Copy
Species: Drosophila melanogaster
Genetic Insert: FOXO
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_52982 Copy
Species: Drosophila melanogaster
Genetic Insert: FOXO
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_52983 Copy
Species: Deinococcus radiodurans
Genetic Insert: L-IFP-F[2]
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pLpC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24747815
Proper citation: RRID:Addgene_52902 Copy
Species: Homo sapiens
Genetic Insert: Reg-L-IFP-F[2]+Venus
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pLpC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24747815
Proper citation: RRID:Addgene_52903 Copy
Species: Deinococcus radiodurans
Genetic Insert: L-IFP-F[1]
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pLpC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24747815
Proper citation: RRID:Addgene_52901 Copy
Species: E.coli
Genetic Insert: OxyS sRNA
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Comments: The transcription of each sRNA was controlled by the pLlacO-1 promoter, which was induced by adding isopropyl-β-d-thiogalactopyranoside (IPTG) to the media. The part of the target mRNA that is necessary for sRNA regulation was fused to gfp and constitutively transcribed from the pLtetO-1 promoter without TetR in the system. GFP fluorescence provides a quantitative measure of target gene expression and sRNA activity.
Proper citation: RRID:Addgene_52989 Copy
Species: Mus musculus
Genetic Insert: Lactaherin
Vector Backbone Description: Backbone Size:3648; Vector Backbone:pPD 49.83; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22727702
Proper citation: RRID:Addgene_52987 Copy
Species: Synthetic
Genetic Insert: nls-ha-MCP-VenusN-nls-ha-PCP-VenusC
Vector Backbone Description: Backbone Size:6200; Vector Backbone:phage; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24402470
Proper citation: RRID:Addgene_52985 Copy
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