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Species: Synthetic
Genetic Insert: sgGFP1, sgCas9
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:2600; Vector Backbone:pENTR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28930690
Proper citation: RRID:Addgene_87908 Copy
Species: Synthetic
Genetic Insert: sgHTT1, shGFP2, sgCas9, cherry
Vector Backbone Description: Backbone Size:7600; Vector Backbone:HIV-1 SIN lentiviral transfer vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28930690
Proper citation: RRID:Addgene_87919 Copy
Species: Synthetic
Genetic Insert: sgHTT1, sgGFP2, shCas9
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:2600; Vector Backbone:pENTR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28930690
Proper citation: RRID:Addgene_87917 Copy
Species: Synthetic
Genetic Insert: pZ-YFP
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pNH603; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27930885
Proper citation: RRID:Addgene_87946 Copy
Species: Synthetic
Genetic Insert: {T_AtHsp18.2}
Vector Backbone Description: Vector Backbone:pBca9145 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27782150
Proper citation: RRID:Addgene_87968 Copy
Species: Synthetic
Genetic Insert: FlpStop
Vector Backbone Description: Backbone Marker:Gene Disruption Project, see Venken et al., 2011, Nat. Methods 8(9): 737-743; Backbone Size:4752; Vector Backbone:pBS-KS-attB1-2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28211790
Proper citation: RRID:Addgene_88910 Copy
Species: Synthetic
Genetic Insert: toxin-antitoxin + LuxABCDE
Vector Backbone Description: Backbone Marker: Nobuhiko Okada (PMID: 9846743); Vector Backbone:pLZ12Km2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24061415
Comments: This plasmid contains a toxin-antitoxin system that stabilizes the plasmid in Streptococcus by post-segregational killing of bacteria that have lost the plasmid. This stabilization improves plasmid utility in animal infection models. For more information, please see the original reference. Although designed for use with Streptococcus pyogenes, this plasmid also labels other streptococcal species. For more information, please see Loh JM and Proft T (2016). PMID: 26410784
Proper citation: RRID:Addgene_88902 Copy
Species: Synthetic
Genetic Insert: toxin-antitoxin + Red-shifted firefly luciferase
Vector Backbone Description: Backbone Marker: Nobuhiko Okada (PMID: 9846743); Vector Backbone:pLZ12Km2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24061415
Comments: This plasmid contains a toxin-antitoxin system that stabilizes the plasmid in Streptococcus by post-segregational killing of bacteria that have lost the plasmid. This stabilization improves plasmid utility in animal infection models. For more information, please see the original reference. Although designed for use with Streptococcus pyogenes, this plasmid also labels other streptococcal species. For more information, please see Loh JM and Proft T (2016). PMID: 26410784
Proper citation: RRID:Addgene_88901 Copy
Species: Synthetic
Genetic Insert: Z3EV promoter cassette
Vector Backbone Description: Backbone Marker:Bahler et al, Yeast, 14(10) 943-51, 1998; Backbone Size:5100; Vector Backbone:pFS6a-KanMX6-P3nmt1; Vector Types:Yeast integration vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28423198
Proper citation: RRID:Addgene_89066 Copy
Species: Synthetic
Genetic Insert: Z3EV promoter GFP-NLS leu1 C-terminal 300 nt
Vector Backbone Description: Backbone Marker:Apolinario, E., Nocero, M., Jin, M., & Hoffman, C. S. (1993). Current Genetics, 24(6), 491–495.; Backbone Size:5763; Vector Backbone:pBSSK-His7(F); Vector Types:S. pombe his7 integration vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28423198
Proper citation: RRID:Addgene_89065 Copy
Species: Synthetic
Genetic Insert: Z3EV artificial transcription factor
Vector Backbone Description: Backbone Marker:Rhind Lab; Backbone Size:5285; Vector Backbone:pFS181 (pSS1); Vector Types:leu1 integrating vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28423198
Proper citation: RRID:Addgene_89064 Copy
Species: Synthetic
Genetic Insert: C-BLa 1.2 fusion protein
Vector Backbone Description: Backbone Marker:John E. Cronan; Backbone Size:4232; Vector Backbone:pBAD322K (modified); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28266525
Comments: TMD: Integrin alphaV mut GP, contains ApaI restriction site
Proper citation: RRID:Addgene_89000 Copy
Species: Synthetic
Genetic Insert: N-BLa 1.1 fusion protein
Vector Backbone Description: Backbone Size:4083; Vector Backbone:pACYC184 (modified); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28266525
Comments: TMD: Integrin alphaV mut GP, contains ApaI restriction site
Proper citation: RRID:Addgene_88994 Copy
Species: Synthetic
Genetic Insert: His6-(pos16)-GFP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5152; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22840771
Proper citation: RRID:Addgene_89249 Copy
Species: Synthetic
Genetic Insert: His6-(pos15)-GFP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5152; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22840771
Proper citation: RRID:Addgene_89248 Copy
Species: Synthetic
Genetic Insert: His6-(pos16)-GFP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5152; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22840771
Proper citation: RRID:Addgene_89252 Copy
Species: Synthetic
Genetic Insert: His6-(pos16)-GFP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5152; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_89250 Copy
Species: Synthetic
Genetic Insert: sf-GFP-GGS9-Cre-6xHis
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5073; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_89343 Copy
Species: Synthetic
Genetic Insert: Cpf1, RR variant
Vector Backbone Description: Backbone Size:4328; Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28581492
Comments: This variant also recognizes CCCC and ACCC PAMs (and, with lower efficiency, VYCV).
Contains a U6-crRNA expression cassette (BbsI restriction sites for guide cloning).
For more information on Zhang lab CRISPR plasmids, please refer to www.addgene.org/crispr/zhang/.
Proper citation: RRID:Addgene_89352 Copy
Species: Synthetic
Genetic Insert: Cpf1, RR variant
Vector Backbone Description: Backbone Size:5402; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28581492
Comments: For more information on Zhang lab CRISPR plasmids, please refer to www.addgene.org/crispr/zhang/.
Proper citation: RRID:Addgene_89355 Copy
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