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Species:
Genetic Insert: OpLac2-Δ6 strain
Vector Backbone Description: Vector Backbone:na; Vector Types:; Bacterial Resistance:None
References:
Comments: Oplac2Δ6 was erroneously synthesized missing the first 6 nucleotides of Oplac2. These deletions correspond to the first 2 N-terminal amino acid residues (Methionine and Threonine), and instead begin at the Methionine at position 3.
Proper citation: RRID:Addgene_52700 Copy
Species:
Genetic Insert: Oplac1 (37)-KI strain
Vector Backbone Description: Vector Backbone:na; Vector Types:; Bacterial Resistance:None
References:
Comments: To test whether the mRNA secondary structure of our codon redesigned sequences affected expression, we substituted the first 37 nucleotides of KILac with those of OpLac1.
Proper citation: RRID:Addgene_52701 Copy
Species:
Genetic Insert: S56L strain
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
References:
Comments: Impaired LacY function
Proper citation: RRID:Addgene_52709 Copy
Species:
Genetic Insert: ΔZ strain
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
References:
Comments: lacZ deletion
Proper citation: RRID:Addgene_52706 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pSILENCER; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments: EGFP is a transfection marker, not a fusion.
Proper citation: RRID:Addgene_52675 Copy
Species: Rattus norvegicus
Genetic Insert: PSD-95
Vector Backbone Description: Backbone Marker:Evrogen; Backbone Size:4733; Vector Backbone:pTagRFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: HindII and EcoRI were used to cut PSD-95 from PSD-95-pGW1 (received from David Bredt) and ligate it into pTagRFP-N. PSD-95 tyrosine 12 was mutated to glutamate (Y12E) by extending a complementary set of primers (PSD95Y12E-forward: 5’-GAC AAC CAA GAA AGA GCG CTA CCA AGA TGA AGA CAC G-3’, PSD95Y12E-reverse: 5'-CGT GTC TTC ATC TTG GTA GCG CTC TTT CTT GGT TGT C-3') along the whole template plasmid using Q5 high-fidelity DNA polymerase (NEB). DpnI was subsequently used to digest non-mutated, methylated template DNA prior to transformation.
Proper citation: RRID:Addgene_52672 Copy
Species: Homo sapiens
Genetic Insert: LIN-41
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52716 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN4
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 493–511 from the start sequence of rat α-actinin-4 (the sense chain was 5’-gactatccaggagatgcagttcaagagactgcatctcctggatagtctttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52679 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN1
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 476–494 from the start sequence of rat α-actinin-1 (the sense chain was 5’-gatctccaacgtcaacaagttcaagagacttgttgacgttggagatctttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52676 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN2
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 224–242 from the start sequence of rat α-actinin-2 (the sense chain was 5’-tggcctgatggatcatgagttcaagagactcatgatccatcaggccatttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52677 Copy
Species:
Genetic Insert: G794D strain
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
References:
Comments:
Proper citation: RRID:Addgene_52710 Copy
Species: Homo sapiens
Genetic Insert: LIN-41
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52717 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: tetO7-Pcyc1+LINKER
Vector Backbone Description: Vector Backbone:pFA6a-kanMX-tetO-Pcyc1 (41023); Vector Types:Yeast Expression, Promoter replacement; Bacterial Resistance:Ampicillin
References:
Comments: This is the same as the "regular" tetO plasmids (41022, 41023, 41024, 41025, or 41026) with an additional LADYGAG linker. This allows amplification of tagging cassettes from the LINKER vectors using the same pair of primers. For the “regular” vectors the reverse primer is unique to each plasmid.
Proper citation: RRID:Addgene_52685 Copy
Species: Mus musculus
Genetic Insert: ABL1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Encodes isoform IV of mouse c-Abl, which is N-myristylated.
Proper citation: RRID:Addgene_52684 Copy
Species: Homo sapiens
Genetic Insert: HMGA2
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52727 Copy
Species: Homo sapiens
Genetic Insert: EGR1
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52724 Copy
Species: Homo sapiens
Genetic Insert: HPV26 L1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6295; Vector Backbone:pVITRO1-neo-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_52601 Copy
Species: Homo sapiens
Genetic Insert: HPV66 L1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6295; Vector Backbone:pVITRO1-neo-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_52602 Copy
Species: Homo sapiens
Genetic Insert: HPV31 L1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6295; Vector Backbone:pVITRO1-neo-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_52600 Copy
Species: Homo sapiens
Genetic Insert: LIN-41
Vector Backbone Description: Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52721 Copy
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