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Species: Homo sapiens
Genetic Insert: PCNA
Vector Backbone Description: Backbone Size:6037; Vector Backbone:pIRES-puro2b; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20693996
Comments: Note that there are a few minor discrepancies between Addgene's quality control sequence and the author's sequence, but these should not affect function.
Proper citation: RRID:Addgene_26461 Copy
Species: S. mediterranea (planarian)
Genetic Insert: npp-1
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:RNAi, T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26518 Copy
Species: S. mediterranea (planarian)
Genetic Insert: npp-2
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Proper citation: RRID:Addgene_26514 Copy
Species: S. mediterranea (planarian)
Genetic Insert: npy-7
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Proper citation: RRID:Addgene_26516 Copy
Species: S. mediterranea (planarian)
Genetic Insert: npy-6
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26478 Copy
Species: S. mediterranea (planarian)
Genetic Insert: spp-3
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: Addgene's quality control sequence has minor discrepancies with the provided depositor's sequence and NCBI; these do not affect plasmid function.
Proper citation: RRID:Addgene_26510 Copy
Species: S. mediterranea (planarian)
Genetic Insert: spp-6
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Proper citation: RRID:Addgene_26512 Copy
Genetic Insert: pHPRT-DRGFP
Vector Backbone Description: Backbone Size:1480; Vector Backbone:pgkPURO; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11751629
Proper citation: RRID:Addgene_26476 Copy
Species: S. mediterranea (planarian)
Genetic Insert: mpl-2
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26471 Copy
Species: Homo sapiens
Genetic Insert: mTOR
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9933627
Proper citation: RRID:Addgene_26607 Copy
Species: Homo sapiens
Genetic Insert: mTOR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9933627
Proper citation: RRID:Addgene_26606 Copy
Species: Homo sapiens
Genetic Insert: mTOR
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9933627
Proper citation: RRID:Addgene_26608 Copy
Species: Homo sapiens
Genetic Insert: mTOR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9933627
Proper citation: RRID:Addgene_26603 Copy
Species: Homo sapiens
Genetic Insert: mTOR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9933627
Proper citation: RRID:Addgene_26605 Copy
Species: Homo sapiens
Genetic Insert: PP1a
Vector Backbone Description: Backbone Marker:Peti Lab (Addgene #26563); Backbone Size:0; Vector Backbone:RP1B; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18992256
Comments: The backbone is a modified version of pET28 and is also available at Addgene (plasmid #26563)
Proper citation: RRID:Addgene_26566 Copy
Species: Rattus norvegicus
Genetic Insert: calcium channel alpha-1B subunit
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/V5-His ABC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10864934
Comments: Isolated from superior cervical ganglia.
For more information about this plasmid: http://neuroscience.brown.edu/LipscombeLab/homepage/otherpages/clonespage/clonespage1.htm
Also see: GENBANK AF222337
Proper citation: RRID:Addgene_26568 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7_LPB
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:2700; Vector Backbone:pDP19; Vector Types:transcription vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: This construct does not produce perfect pre-let-7. There are leader and tail sequences from the backbone MCS. Used for in vitro translation.
Proper citation: RRID:Addgene_26560 Copy
Species: Rattus norvegicus
Genetic Insert: S6K1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11729323
Proper citation: RRID:Addgene_26610 Copy
Species: Firefly
Genetic Insert: firefly luciferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11114166
Proper citation: RRID:Addgene_26612 Copy
Species: Mus musculus
Genetic Insert: Cacna1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/V5-HisA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18001290
Comments: The fragment was amplified from mouse brain. The GenBank sequence is AY714490.
Proper citation: RRID:Addgene_26578 Copy
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