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Species: Rattus norvegicus
Genetic Insert: Cx 26 (rat)
Vector Backbone Description: Vector Backbone:MTK0_027; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31686495
Comments: Type 3b parts are used for coding sequences in the MTK. Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123796 Copy
Species: Rattus norvegicus
Genetic Insert: GCaMP6s
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:5609; Vector Backbone:pAAV-Ef1a-fDIO hChR2(H134R)-EYFP; Vector Types:Mammalian Expression, AAV, Flp/Frt; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_105714 Copy
Species: Rattus norvegicus
Genetic Insert: synaptophysin-GCaMP6s
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:6109; Vector Backbone:pAAV-EF1a-Flpo; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_105715 Copy
Species: Rattus norvegicus
Genetic Insert: aPKC
Vector Backbone Description: Vector Backbone:pCS2+ hsp70; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25600237
Proper citation: RRID:Addgene_105946 Copy
Species: Rattus norvegicus
Genetic Insert: LAP2b
Vector Backbone Description: Vector Backbone:GW_pDONR_P2r-P3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30120238
Proper citation: RRID:Addgene_105978 Copy
Species: Rattus norvegicus
Genetic Insert: LAP2b
Vector Backbone Description: Vector Backbone:GWpTol2Dest(R4-R3)pA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Proper citation: RRID:Addgene_105980 Copy
Species: Rattus norvegicus
Genetic Insert: aPKC CAAX
Vector Backbone Description: Vector Backbone:GWpTolDest(R4-R2)pA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27810916
Proper citation: RRID:Addgene_105957 Copy
Species: Rattus norvegicus
Genetic Insert: MBP-tagged rat pyruvate kinase liver isoform
Vector Backbone Description: Backbone Size:4467; Vector Backbone:PCRT7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31825824
Proper citation: RRID:Addgene_107157 Copy
Species: Rattus norvegicus
Genetic Insert: MBP-tagged rat pyruvate kinase liver isoform
Vector Backbone Description: Backbone Size:4467; Vector Backbone:PCRT7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31825824
Proper citation: RRID:Addgene_107156 Copy
Species: Rattus norvegicus
Genetic Insert: rat IgG2a Hinge-G4S-Sortag-Histag
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:3956; Vector Backbone:pCR4 TOPO TA; Vector Types:Bacterial Expression, CRISPR, HDR template; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31489367
Proper citation: RRID:Addgene_124810 Copy
Species: Rattus norvegicus
Genetic Insert: fRCaMP2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3703; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651353
Comments: fRCaMP2 is the D396A variant of the jRCaMP1a gene cloned from the pGP-CMV vector (#61563) published by Dana et al. 2016.
Proper citation: RRID:Addgene_125246 Copy
Species: Rattus norvegicus
Genetic Insert: fRCaMP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3955; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30651353
Comments: fRCaMP2 is the D396A variant of the jRCaMP1a gene cloned from the pGP-CMV vector (#61563) published by Dana et al. 2016.
Proper citation: RRID:Addgene_125250 Copy
Species: Rattus norvegicus
Genetic Insert: CCAAT/Enhancer-binding Protein beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The C/EBPbeta gene encodes 3 in-frame translational isoforms (LAP*, LAP, and LIP). LAP is the predominant activating isoform expressed in most cells, and this plasmid is designed to express the LAP isoform of the protein. The construct does not include the extra 21 codons at the N terminus that are present in LAP*. The complete insert (not just the ORF) includes a Kozak box sequence at the N terminus.
For more information on the C/EBPbeta isoforms, please see the original reference:
Descombes, P., and Schibler, U. A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569-579 (1991).
Proper citation: RRID:Addgene_12558 Copy
Species: Rattus norvegicus
Genetic Insert: CCAAT/Enhancer-binding Protein alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12578822
Proper citation: RRID:Addgene_12550 Copy
Species: Rattus norvegicus
Genetic Insert: Neu
Vector Backbone Description: Backbone Marker:Yi Li; Vector Backbone:RCAS-Y; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17090666
Proper citation: RRID:Addgene_125832 Copy
Species: Rattus norvegicus
Genetic Insert: SEPT11
Vector Backbone Description: Vector Backbone:pCDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19380581
Proper citation: RRID:Addgene_120315 Copy
Species: Rattus norvegicus
Genetic Insert: SEPT11
Vector Backbone Description: Vector Backbone:pEGFP N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19380581
Proper citation: RRID:Addgene_120316 Copy
Species: Rattus norvegicus
Genetic Insert: SEPT11
Vector Backbone Description: Vector Backbone:PEF6-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19380581
Proper citation: RRID:Addgene_120318 Copy
Species: Rattus norvegicus
Genetic Insert: GABRG2 shRNA
Vector Backbone Description: Vector Backbone:mU6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16248887
Comments: It targets gamma2 GABAA receptor subunit mRNA, nucleotides 130–155 located in the protein coding region of GenBank accesion number L08497. It targets both gamma2S and gamma2L mRNAs).
Proper citation: RRID:Addgene_120794 Copy
Species: Rattus norvegicus
Genetic Insert: GABRG2 shRNA
Vector Backbone Description: Vector Backbone:mU6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16248887
Comments: 3-point mutations (negative control for shRNA2 targeting gamma2 mRNA, plasmid 120794).
Proper citation: RRID:Addgene_120795 Copy
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