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Species: Rattus norvegicus
Genetic Insert: MAPK1
Vector Backbone Description: Backbone Marker:David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17114285
Proper citation: RRID:Addgene_40815 Copy
Species: Rattus norvegicus
Genetic Insert: β-arrestin 1 S412D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9924018
Comments: Flag epitope-tagged truncation mutants of β-arrestin 1 were prepared by the polymerase chain reaction (PCR), incorporating an Eco RI site, minimal Kozak sequence (ACC), and initiator methionine codon into the 5' primer, and the Flag epitope sequence, stop codon, and an Xho I site into the 3' primer. PCR products were subcloned into pcDNA3. DNA sequences were confirmed by dideoxynucleotide sequencing.
Proper citation: RRID:Addgene_42196 Copy
Species: Rattus norvegicus
Genetic Insert: rM3D-mCherry
Vector Backbone Description: Backbone Size:4818; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_50458 Copy
Species: Rattus norvegicus
Genetic Insert: mRuby2-P2A-GCaMP6m
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27284193
Comments: The discrepancies between the full sequence and Addgene's QC sequence should not have any functional consequence.
Proper citation: RRID:Addgene_51473 Copy
Species: Rattus norvegicus
Genetic Insert: Chimeric Synaptophysin-mCherry-Luciferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24529383
Proper citation: RRID:Addgene_51819 Copy
Species: Rattus norvegicus
Genetic Insert: MIBP1
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22294689
Proper citation: RRID:Addgene_51870 Copy
Species: Rattus norvegicus
Genetic Insert: MIBP1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11872163
Comments: Makino, R., Akiyama, K., Yasuda, J., Mashiyama, S., Honda, S., Sekiya, T., & Hayashi, K. (1994). Cloning and characterization of a c-myc intron binding protein (MIBP1). Nucleic acids research, 22(25), 5679-5685.
Proper citation: RRID:Addgene_51872 Copy
Species: Rattus norvegicus
Genetic Insert: GAL4(1-147)-CREB-S133A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4767; Vector Backbone:pcDNAI-AMP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7958915
Proper citation: RRID:Addgene_46770 Copy
Species: Rattus norvegicus
Genetic Insert: GAL4(1-147)-CREB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4767; Vector Backbone:pcDNAI-AMP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7958915
Proper citation: RRID:Addgene_46769 Copy
Species: Rattus norvegicus
Genetic Insert: soluble guanylate cyclase 1alpha3
Vector Backbone Description: Backbone Marker:Homemade; Backbone Size:6642; Vector Backbone:pMA3211; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22637478
Comments: Please note that though there are some differences between Addgene's quality control sequence and the depositor's assembled sequence, this plasmid should function as reported.
Proper citation: RRID:Addgene_46874 Copy
Species: Rattus norvegicus
Genetic Insert: soluble guanylate cyclase1 beta 3
Vector Backbone Description: Backbone Size:5684; Vector Backbone:pMA1662; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22637478
Proper citation: RRID:Addgene_46877 Copy
Species: Rattus norvegicus
Genetic Insert: MAP4K3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11384986
Proper citation: RRID:Addgene_47402 Copy
Species: Rattus norvegicus
Genetic Insert: SAPAP1
Vector Backbone Description: Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9115257
Proper citation: RRID:Addgene_47940 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11060023
Proper citation: RRID:Addgene_45244 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11060023
Proper citation: RRID:Addgene_45245 Copy
Species: Rattus norvegicus
Genetic Insert: PKA regulatory subunit RII beta
Vector Backbone Description: Backbone Marker:Karel Svoboda; Backbone Size:5900; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, with chicken beta actin promoter and WPRE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19447092
Proper citation: RRID:Addgene_45529 Copy
Species: Rattus norvegicus
Genetic Insert: NR1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12160751
Proper citation: RRID:Addgene_45446 Copy
Species: Rattus norvegicus
Genetic Insert: NR2A
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12160751
Proper citation: RRID:Addgene_45445 Copy
Species: Rattus norvegicus
Genetic Insert: AT1AR-TSTS/A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15355986
Comments: Mutant AT1A receptors were generated by mutagenesis PCR using pcDNA3.1-HA-AT1A receptor (provided by M. G. Caron, Duke University) as a template and a QuikChange multisite-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions. The primer for the TSTS/A receptor was:
5' p-CTCAAGCCTGTCTGCGAAAATGGCCGCTTGCTTACCGGCCTTC 3'.
N4D mutation introduced to prevent glycosylation at this consensus site and steric hindrance of antibody binding.
Proper citation: RRID:Addgene_45634 Copy
Species: Rattus norvegicus
Genetic Insert: α1Ic
Vector Backbone Description: Backbone Marker:Perez-Reyes; Backbone Size:5400; Vector Backbone:pcDNA3-HE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12297319
Proper citation: RRID:Addgene_45808 Copy
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