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Hendrik F Van Essen 2019. DNA isolation from Formalin-Fixed, Paraffin-Embedded (FFPE) material. protocols.io https://dx.doi.org/10.17504/protocols.io.zjwf4peCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.zjwf4pe
Authors: Hendrik F Van Essen
Summary: This protocol describes the isolation of DNA from paraffin embedded tissue. The resulting DNA can be used for CGH array hybridizations. After removal of the paraffin, the tissue is lysed and the DNA is released from the cells. Then the DNA is isolated by using a silica-based column that binds the DNA.
Associated Publications: Vincenten JPL, Essen HFv, Lissenberg-Witte BI, Bulkmans NWJ, Krijgsman O, Sie D, Eijk PP, Smit EF, Ylstra B, Thunnissen E (2019) Clonality analysis of pulmonary tumors by genome-wide copy number profiling. PLoS ONE 14(10): e0223827. doi: 10.1371/journal.pone.0223827
Affiliations: Amsterdam UMC
External URL: https://doi.org/10.1371/journal.pone.0223827
Version: 1
Publication Date: 2019
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