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URL: https://dx.doi.org/DOI:10.17504/protocols.io.xy4fpyw

Authors: Daniel Gyllborg, Mats Nilsson

Group: Human Cell Atlas Method Development Community, Molecular Diagnostics - Mats Nilsson Group, Neurodegeneration Method Development Community

Summary: Protocol for multiplexed in situ sequencing in tissue sections as an image-based spatial transcriptomic method. This is the second iteration of In Situ Sequencing (HybISS: Hybridization based In Situ Sequencing) based on the principles published in Ke et al. Nature Methods, 2013 and more recently Qian et al. Nature Methods, 2019. Here we present a modified method in detection chemistry using sequencing by hybridization approach for combinatorial decoding. This results in a more robust method for detecting larger probe panels for a more high-throughput in situ sequencing method.

Associated Publications: Gyllborg D, Langseth CM, Qian X, Choi E, Salas SM, Hilscher MM, Lein ES, Nilsson M, Hybridization-based sequencing (HybISS) for spatially resolved transcriptomics in human and mouse brain tissue. Nucleic Acids Research 48(19). doi: 10.1093/nar/gkaa792

Affiliations: Department of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University, Solna, Sweden, Department of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University, Solna, Sweden

External URL: https://doi.org/10.1093/nar/gkaa792

Version: 1

Publication Date: 2020

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