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Arnav Moudgil, Robi D. Mitra 2019. Single Cell Calling Cards Library Preparation. protocols.io https://dx.doi.org/10.17504/protocols.io.xwifpceCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.xwifpce
Authors: Arnav Moudgil, Robi D. Mitra
Group: Human Cell Atlas Method Development Community, Transposon Calling Cards
Summary: This protocol describes how to create calling card libraries from single cell RNA. We assume you have successfully transformed cells with piggyBac self-reporting transposons and either undirected piggyBac transposase or your favorite transcription factor (YFTF) fused to piggyBac. We also assume you have optimized the dissociation protocol for your specific cells or tissues and can generate single cell suspensions.
Affiliations: Washington University, Saint Louis, Washington University, Saint Louis
External URL: https://doi.org/10.1101/538553
Version: 1
Publication Date: 2019
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