Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Jason Kim 2019. U Mass - Adiponectin. protocols.io https://dx.doi.org/10.17504/protocols.io.xm9fk96Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.xm9fk96
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: The adiponectin enzyme-linked immunosorbent assay (ELISA) is a standard immunoassay for the detection of total adiponectin levels in serum, adipocyte extracts, and cell culture media samples. This experiment uses a 96-well plate coated with anti-adiponectin antibodies and developed with a secondary anti-adiponectin antibody linked to horseradish peroxidase. The ELISA is developed with 3,3',5,5'-Tetramethylbenzidine (TMB), a chromogenic substrate and read on an automated plate spectrophotometer at 450 nm. Adiponectin is an adipocyte-derived hormone (adipokine) that regulates insulin sensitivity and glucose metabolism.
RRIDs used: RRID:AB_2651034
Affiliations: University of Massachusetts
External URL: https://mmpc.org/shared/document.aspx?id=165&docType=Protocol
Version: 1
Publication Date: 2019
Expand AllComing soon.
Check
for resource mentions.
Coming soon.
Source:
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
