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Monserrat López-Sanmartín, Gaetano Catanese, Amalia Grau, Jose María Valencia, Jose Rafa García-March, José Ignacio Navas 2019. Species-specific quantitative PCR (qPCR) protocol developed to identify Haplosporidium pinnae. protocols.io https://dx.doi.org/10.17504/protocols.io.xe7fjhnCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.xe7fjhn
Authors: Monserrat López-Sanmartín, Gaetano Catanese, Amalia Grau, Jose María Valencia, Jose Rafa García-March, José Ignacio Navas
Summary: The aim of this study has been to develop species-specific quantitative PCR (qPCR) protocol carrying out a fast, specific and effective molecular diagnose of Haplosporidium pinnae. In this sense, the detection limit for qPCR was equal to 30 copies of SSU rDNA / ng of DNA using plasmid alone and when 100ng DNA of non-infected oyster were added.
Associated Publications: López-Sanmartín M, Catanese G, Grau A, Valencia JM, García-March JR, Navas JI (2019) Real-Time PCR based test for the early diagnosis of Haplosporidium pinnae affecting fan mussel Pinna nobilis. PLoS ONE 14(2): e0212028. doi: 10.1371/journal.pone.0212028
Affiliations: IFAPA Agua del Pino, LIMIA-INAGEA, LIMIA-INAGEA, LIMIA-INAGEA, IMEDMAR-UCV , IFAPA Agua del Pino
External URL: https://doi.org/10.1371/journal.pone.0212028
Version: 1
Publication Date: 2019
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