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Ian Mackay, Judy Northill 2019. Enterovirus (EV) D68 real-time RT-PCR (EV-D68-TM2018). protocols.io https://dx.doi.org/10.17504/protocols.io.xb4fiqwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.xb4fiqw
Authors: Ian Mackay, Judy Northill
Group: Public Health Virology, Forensic and Scientific Services
Summary: This protocol aims to amplify enterovirus (EV) D68 viruses but not other viruses.This protocol is modified from a previously published method cited below. Details of the modification are included in the method.The oligonucleotides target the 5'UTR noncoding region. This is a qualitative test for investigating EV-D68 infection of humans.The test has identified both the historical EV-D68 strains and contemporary strains which has been confirmed by subgenomic sequencing of partial 5'UTR-VP2 and partial VP1 regions. Culture of the virus is not required as this assay is capable of detecting EV-D68 direct from extracted clinical samples.
Affiliations: Public Health Virology, Forensic and Scientific Services, Public Health Virology, Forensic and Scientific Services
External URL: https://www.ncbi.nlm.nih.gov/pubmed/25542461
Version: 2
Publication Date: 2019
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