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Thuc Nguyen, Emma Garren 2019. Single molecule FISH. protocols.io https://dx.doi.org/10.17504/protocols.io.xb2fiqeCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.xb2fiqe
Authors: Thuc Nguyen, Emma Garren
Group: CZI Spatial Transcriptomics Protocol Repository
Summary: This protocol describes multiround hybrization of directly-conjugated FISH probes for single molecule RNA detection. Thin tissue sections (10-μm) are placed onto silanized coverslips (24x50) that fit onto an ASI imaging chamber. A SecureSeal chamber is placed around the sections, which act as a reaction chamber and imaging chamber.
Affiliations: Allen Institute for Brain Science, Allen Institute for Brain Science
Version: 1
Publication Date: 2019
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