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Laure-Emmanuelle Zaragosi, Pascal Barbry 2019. Cell dissociation from airway biopsies with cold-active protease for single-cell RNA-seq. protocols.io https://dx.doi.org/10.17504/protocols.io.x3efqjeCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.x3efqje
Authors: Laure-Emmanuelle Zaragosi, Pascal Barbry
Group: Human Cell Atlas Method Development Community
Summary: This protocol provides details on the cell dissociation that should be performed to obtain single-cell suspensions from airway biopsies.Biopsies may come from tracheal, bronchial or nasal epithelium.Cell dissociation is performed at 4°C to avoid gene expression alterations and maximize viability. The typical cell number recovery is 40 000 cells for one biopsy.Cell suspensions are suitable for single-cell RNA-sequencing protocols.
Affiliations: Université Côte d'Azur, CNRS, IPMC, 06560 Valbonne, France, Université Côte d'Azur, CNRS, IPMC, 06560 Valbonne, France
Version: 1
Publication Date: 2019
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