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Binnypreet Kaur1, 2, Drahomíra Faktorová1, 2 and Julius Lukeš1, 2, 3 2019. Transfectionof constructs in diplonemids to block NHEJ pathway using the inhibitors of KU70/80 proteins.. protocols.io https://dx.doi.org/10.17504/protocols.io.x35fqq6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.x35fqq6
Authors: Binnypreet Kaur1, 2, Drahomíra Faktorová1, 2 and Julius Lukeš1, 2, 3
Group: Protist Research to Optimize Tools in Genetics (PROT-G), Julius Lukes
Summary: The process of NHEJ is completed when the DNA ligase complex ligates the broken ends of the DNA. Ku70-Ku80 are the DNA-dependent protein kinase heterodimeric regulatory factor that forms a link between two broken DNA ends and structurally support, align and protect them from further degradation. W7 inhibitor (W7(N-(6-aminohexyl)-5-chloro1-naphthalenesulfonamide) inhibits the cofactor of inositol hexakisphophate which effects the regulatory factor of Ku heterodimers.
Affiliations: Institute of Parasitology, Czech Academy of Sciences, Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic, Institute of Parasitology, Czech Academy of Sciences, Institute of Parasitology, Czech Academy of Sciences3, Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic, Canadian Institute for Advanced Research, Toronto, Canada
Version: 2
Publication Date: 2019
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