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Michael Frimpong, Hubert Senanu Ahor 2019. Recombinase polymerase amplification assay for detection of Mycobacterium ulcerans DNA. protocols.io https://dx.doi.org/10.17504/protocols.io.vvve666Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.vvve666
Authors: Michael Frimpong, Hubert Senanu Ahor
Summary: This document describes the standard operating procedure for the application of the real time Mu-RPA assay with Exo probe system. The assay detects M. ulcerans DNA (IS2404) from clinical sample or culture suspension. The assay consists of recombinase polymerase amplification TwistDx Exo kit procedure. The reaction mix must be prepared in an environment free of DNA amplicons. Personal protective clothing (i.e. lab coats, gloves) must be used throughout the process.
Associated Publications: Frimpong M, Ahor HS, Wahed AAE, Agbavor B, Sarpong FN, Laing K, Wansbrough-Jones M, Phillips RO (2019) Rapid detection of Mycobacterium ulcerans with isothermal recombinase polymerase amplification assay. PLoS Negl Trop Dis 13(2): e0007155. doi: 10.1371/journal.pntd.0007155
Affiliations: Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi Centre for Collaborative Research in Tropical Medicine
External URL: https://doi.org/10.1371/journal.pntd.0007155
Version: 1
Publication Date: 2019
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