Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Paola Marcovecchio, Sara Mcardle, Zbigniew Mikulski, Angela Denn, Katarzyna Dobaczewska 2018. Mouse Tissue Fixation with Paraformaldehyde for Fluorescent Reporter Mice. protocols.io https://dx.doi.org/10.17504/protocols.io.vnge5bwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.vnge5bw
Authors: Paola Marcovecchio, Sara Mcardle, Zbigniew Mikulski, Angela Denn, Katarzyna Dobaczewska
Group: Optical Clearing of Tissue, La Jolla Institute Microscopy Core
Summary: Fixation of mouse tissues for either histology or fluorescence microscopy is an essential first step in tissue preparation. In this protocol, we outline a reliable method for fixation and perfusion of mice for tissue histology or microscopy. Tissues prepared by this method can be used downstream as FFPE sections, cryosections for fluorescence microscopy, or tissue clearing for whole tissue imaging. For mice with transgenic fluorescent reporters, it is especially important that the tissues are preserved properly to ensure that the signal of the fluorescent reporter can be imaged.
Affiliations: La Jolla Institute for Immunology, La Jolla Institute for Immunology, La Jolla Institute for Immunology, La Jolla Institute for Allergy and Immunology, La Jolla Institute for Allergy and Immunology
Version: 2
Publication Date: 2018
Expand AllComing soon.
Check
for resource mentions.
Coming soon.
Source:
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
