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URL: https://dx.doi.org/DOI:10.17504/protocols.io.vdse26e

Authors: Tiffany Borbon, Corey Parlet, Gwendolyn Clay, Mary E. Wilson

Summary: Cutaneous coinfection of bacteria with Leishmania species in mice provides a model for studying the immune effects of non-sterile inoculation of Leishmania protozoa. In this protocol, we describe a method for intradermal coinfection of mice ear pinnas with Leishmania major IA-2 and a low dose of Staphylococcus aureus Newman strain. This protocol describes the methods for S. aureus and L. major preparation, mixing, mouse anesthesia and intradermal injection as well as infection monitoring and euthanasia. This protocol can be modified for the co-inoculation of other bacterial or leishmanial species in mice.

Associated Publications: Borbón TY, Scorza BM, Clay GM, Queiroz FLNd, Sariol AJ, Bowen JL, Chen Y, Zhanbolat B, Parlet CP, Valadares DG, Cassel SL, Nauseef WM, Horswill AR, Sutterwala FS, Wilson ME (2019) Coinfection with Leishmania major and Staphylococcus aureus enhances the pathologic responses to both microbes through a pathway involving IL-17A. PLoS Negl Trop Dis 13(5): e0007247. doi: 10.1371/journal.pntd.0007247

Affiliations: University of Iowa, University of Iowa, University of California San Diego, University of Iowa

External URL: https://doi.org/10.1371/journal.pntd.0007247

Version: 1

Publication Date: 2018

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