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Sebastien Colin 2018. Simple 3D imaging of biogenic silica structures by fluorescent microscopy. protocols.io https://dx.doi.org/10.17504/protocols.io.vd8e29wCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.vd8e29w
Authors: Sebastien Colin
Group: Ecology of Marine Plankton (ECOMAP) team - Roscoff, Tara Oceans
Summary: This protocol describes how to prepare, stain and mount biogenic silica particles (opaline from diatoms, Radiolaria, sponges spicules, phytolith...) for 3D imaging with fluorescent microscopy. The protocol is fast, convenient and inexpensive. Hence it could be a relevant alternative to X-ray computed tomography or photogrammetry.
Affiliations: CNRS, Station Biologique de Roscoff, team Evolution of Plankton and Pelagic Ecosystems (C. de Vargas)
Version: 1
Publication Date: 2018
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