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Rodolfo Salido Benitez 2018. 16S Illumina Primer Plate Aliquoting (working plates 5µM Forw & Rev). protocols.io https://dx.doi.org/10.17504/protocols.io.uz7ex9nCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.uz7ex9n
Authors: Rodolfo Salido Benitez
Summary: The following protocol outlines aliquoting resuspended primers, stored at 100µM in stock plates, into working primer plates at a conentration of 5µM for both forward (barcoded) and reverse primers, using the epMotion 5075. The Knigh Lab stores primer combos premixed in working primer plates.
Associated Publications: Minich JJ, Humphrey G, Benitez RAS, Sanders J, Swafford A, Allen EE, Knight R, High-Throughput Miniaturized 16S rRNA Amplicon Library Preparation Reduces Costs while Preserving Microbiome Integrity. mSystems 3(6). doi: 10.1128/mSystems.00166-18
Affiliations: Knight Lab @ UC San Diego Health
External URL: https://doi.org/10.1128/mSystems.00166-18
Version: 1
Publication Date: 2018
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