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Blue Lake, Kun Zhang 2019. Isolation of single nuclei from solid tissues. protocols.io https://dx.doi.org/10.17504/protocols.io.ufketkwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.ufketkw
Authors: Blue Lake, Kun Zhang
Group: Human Cell Atlas Method Development Community, KPMP, Human BioMolecular Atlas Program (HuBMAP) Method Development Community
Summary: Nuclei can be readily isolated from frozen tissues with a combination of chemical and physical treatments that can circumvent the non-uniform or incomplete dissociation of solid tissues into single cells. The isolation of nuclei can also circumvent RNA degradation or any introduction of technical artefacts (such as stress responses) that could be triggered during whole cell dissociation methods. Data generated from single-nucleus genomic assays permits discovery of molecular cell types that can be used to define the overall cellular makeup of a tissue or organ, and ultimately will inform upon adult human tissue atlases.
Affiliations: University of California, San Diego, University of California, San Diego
External URL: http://genome-tech.ucsd.edu/ZhangLab/
Version: 1
Publication Date: 2019
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