Resource Summary Report  New Search Previous Search Results

URL: https://dx.doi.org/DOI:10.17504/protocols.io.tv3en8n

Authors: Alexandra Ehl, David Frommholz, Nadine Stefanczyk

Summary: Purification Guide for the Isolation of Histidine-tagged Proteins with ChroSpin Columns by DALEX Biotech.ChroSpin-IMAC by DALEX Biotech offers a robust and convenient way to isolate polyhistidine-tagged proteins from bacterial, mammalian, and insect cell cultures.Immobilized metal affinity chromatography (IMAC) is based on the interaction of the imidazole ring of histidine with transition metal ions immobilized on a solid support. Recombinant proteins with a 3 - 10 histidine fusion tag bind to these metal ions while unwanted proteins are removed by washing with excess binding buffer. Elution of the target protein is achieved by the addition of imidazole, EDTA or a low pH. Easy and quick small scale fusion protein purification from various sources.Low metal leaching from column.Tolerates reducing reagents (e.g. DTT up to 10 mM).The proprietary resin does not shrink or swell in aqueous buffers.High pressure stability.pH stability short term 2 - 8, long term 3 - 8.Excellent thermal stability up to 15 minutes at 120 °C in aqueous buffers at neutral pH.Can be dried for long term storage (80 °C for > 2 h).

Affiliations: DALEX Biotech, DALEX Biotech, DALEX Biotech

External URL: https://dalex-biotech.com

Version: 1

Publication Date: 2018

Expand All Collapse