Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Kelsey Knight 2018. Immunohistochemistry Protocol for Frozen Sections. protocols.io https://dx.doi.org/10.17504/protocols.io.tkfektnCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.tkfektn
Authors: Kelsey Knight
Group: BioLegend
Summary: The following is a general procedure guide for preparation and staining of acetone-fixed frozen tissues using a purified, unconjugated primary antibody, biotinylated secondary antibody and streptavidin-horseradish peroxidase (Sav-HRP) and DAB detection system. Because each antigen differs in terms of requirement for fixation, amplification step, etc., it is not possible to write an inclusive protocol that will work for all antigens. The user must determine optimal conditions for each antigen of interest. Many protocols for staining individual antigens, as well as useful tips and troubleshooting guides for immunohistochemistry, can be found at the IHC World web site (http://www.ihcworld.com/).
Affiliations: BioLegend
External URL: https://www.biolegend.com/protocols/immunohistochemistry-protocol-for-frozen-sections/4288/
Version: 2
Publication Date: 2018
Expand AllComing soon.
Check
for resource mentions.
Coming soon.
Source:
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
