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Eric Deflesselle 2018. METADAP genetic protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.ti2ekgeCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.ti2ekge
Authors: Eric Deflesselle
Summary: Genomic DNA was extracted from circulating blood leucocytes by using Gentra Puregene Blood Kits according to the manufacturer’s protocol (Qiagen) and was stored at -20°C. The TRKB polymorphismswere genotyped by the IntegraGen company (Evry, France) using the Fluidigm®BioMarkTMHD system (Fluidigm Corporation, CA, USA), using TaqMan allelic discrimination (Coulbault et al., 2006), with the ABI Prism® 7900HT Sequence Detection System (Life Technologies).
Associated Publications: Deflesselle E, Colle R, Rigal L, David DJ, Vievard A, Martin S, Becquemont L, Verstuyft C, Corruble E (2018) The TRKB rs2289656 genetic polymorphism is associated with acute suicide attempts in depressed patients: A transversal case control study. PLoS ONE 13(10): e0205648. doi: 10.1371/journal.pone.0205648
Affiliations: Université Paris Sud (Paris XI)
External URL: https://doi.org/10.1371/journal.pone.0205648
Version: 1
Publication Date: 2018
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