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Cleyde Helena 2018. Acrolein + PFA perfusion for immunocytochemistry . protocols.io https://dx.doi.org/10.17504/protocols.io.tb9eir6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.tb9eir6
Authors: Cleyde Helena
Summary: Appropriate tissue fixation is essential for a good quality immunocytochemistry (ICC).There are several fixation methods, but "whole body" or "target" perfusion of the animal is one of the most efficient methods.Most perfusing protocols include only paraformaldehyde, but acrolein penetrates tissue more rapidly, improving final fixation of the tissue.
Affiliations: ASC group
Version: 1
Publication Date: 2018
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