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Protocol Name
Angiotensin-converting enzyme inhibitory assay
DOI:DOI:10.17504/protocols.io.tb4eiqw RRID Copied  
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Francisco Herrera 2018. Angiotensin-converting enzyme inhibitory assay. protocols.io https://dx.doi.org/10.17504/protocols.io.tb4eiqw
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.tb4eiqw

Authors: Francisco Herrera

Summary: The use of extracts obtained from various plants is an activity developed for years with the purpose of taking advantage of resources from the regions to prevent or cure a specific disease. On the other hand, the application of in vitro techniques, as angiotensin-converting enzyme inhibitory assay have allowed finding the relationship of organic compounds with biological activities and thus be able to determine which type of plants or their extracts are suitable to be used as a treatment for hypertension. In this protocol is described a method based on the colorimetric reaction of the hippuric acid with trichloro-triazine (TT).

Associated Publications: Rodríguez-García CM, Ruiz-Ruiz JC, Peraza-Echeverría L, Peraza-Sánchez SR, Torres-Tapia LW, Pérez-Brito D, Tapia-Tussell R, Herrera-Chalé FG, Segura-Campos MR, Quijano-Ramayo A, Ramón-Sierra JM, Ortiz-Vázquez E (2019) Antioxidant, antihypertensive, anti-hyperglycemic, and antimicrobial activity of aqueous extracts from twelve native plants of the Yucatan coast. PLoS ONE 14(3): e0213493. doi: 10.1371/journal.pone.0213493

Affiliations: Instituto Tecnológico Superior Progreso

External URL: https://doi.org/10.1016/0003-2697(78)90053-2

Version: 1

Publication Date: 2018

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