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Protocol Name
Modified ZN Staining Protocol
DOI:DOI:10.17504/protocols.io.tb2eiqe RRID Copied  
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Asar Khan, Sumaira Shams, Saima Khan, Muhammad Iftikhar Khan, Sardar Khan, Abid Ali 2018. Modified ZN Staining Protocol . protocols.io https://dx.doi.org/10.17504/protocols.io.tb2eiqe
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.tb2eiqe

Authors: Asar Khan, Sumaira Shams, Saima Khan, Muhammad Iftikhar Khan, Sardar Khan, Abid Ali

Summary: The Modified Ziehl-Neelsen stain (mZN stain) is a type of differential bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria. Acid fast organisms are those which are capable of retaining the primary stain when treated with an acid (fast=holding capacity). Members of the Actinomycetes, genus Nocardia (N. brasiliensis and N. asteroides are opportunistic pathogens) are partially acid-fast. Oocysts of coccidian parasites, such as Cryptosporidium and Isospora, are also acid-fast. Hence they can also be detected and identified through mZN staining procedure.

Affiliations: Department of Zoology Abdul Wali Khan University Mardan, KPK Pakistan, Department of Zoology Abdul Wali Khan University Mardan, KPK Pakistan, Department of Zoology Abdul Wali Khan University Mardan, KPK Pakistan, Centre for Biotechnology and Microbiology University of Swat, Pakistan, Department of Environmental Science, University of Peshawar, Department of Zoology Abdul Wali Khan University Mardan, KPK Pakistan

Version: 2

Publication Date: 2018

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