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David Eccles 2018. Stranded Mapping from Long Reads. protocols.io https://dx.doi.org/10.17504/protocols.io.syheft6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.syheft6
Authors: David Eccles
Summary: This protocol demonstrates how to convert raw long reads produced using a strand-specific sequencing protocol (e.g. ONT's strand-switching protocol) into strand-specific mapped reads.The general ides is to use LAST to identify the adapter orientation relative to the genome, and then use that information to reorient called sequences to create a stranded BAM files that is displayable in a genome browser.
Affiliations: Malaghan Institute of Medical Research (NZ)
External URL: https://bioinformatics.stackexchange.com/a/3922/73
Version: 3
Publication Date: 2018
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