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Nancy Smith, Aliya Spigelman, Haopeng Lin, Patrick Macdonald 2020. Mouse Pancreatic Islet Isolation. protocols.io https://dx.doi.org/10.17504/protocols.io.sqaedseCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.sqaedse
Authors: Nancy Smith, Aliya Spigelman, Haopeng Lin, Patrick Macdonald
Group: CIRTNR2FIC
Summary: This protocol details islet isolation from mouse pancreas. The protocol is divided into 3 main parts; in situ perfusion of pancreas with collagenase, pancreas digestion, and islet purification. A link is provided for the purification using histopque gradient.
Affiliations: University of Alberta, University of Alberta, University of Alberta, University of Alberta
External URL: http://www.bcell.org
Version: 1
Publication Date: 2020
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