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Robert Mahoney 2018. Adult mouse testis cell dissociation (on ice). protocols.io https://dx.doi.org/10.17504/protocols.io.smkec4wCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.smkec4w
Authors: Robert Mahoney
Group: Human Cell Atlas Method Development Community
Summary: This protocol is used to dissociate adult (8-10 week) mouse testis into a single cell suspension. The entire procedure is carried out 'on ice', reducing artifact gene expression changes. The yield is 5.4 million cells from 25 mg tissue and the viability is ~99%.
Affiliations: CCHMC
Version: 1
Publication Date: 2018
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