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URL: https://dx.doi.org/DOI:10.17504/protocols.io.sgmebu6

Authors: Leticia Peraza Echeverría, Cecilia Rodríguez, Dalia Zapata

Summary: Continuous in vitro conidial production from Mycosphaerella fijiensis mycelium. A method for in vitro production of conidia is described. M fijiensis was grown on culture medium containing V8 juice. Mycelium was macerated until a fine suspension was produced; 2 mL was carefully spread on V8 sporulation medium. The Petri dishes were incubated for 6 days at 20 °C, under continuous cool-white fluorescent and black light. After the incubation period, 2.4 mL of 1% gelatin was added to each Petri dish, the conidia carefully dislodged with a camel hair brush. Conidial production was notably stimulated by brush harvesting mycelial cultures. After 6 days of culture the first harvest was made and the same batch of petri dishes were put in the same culture conditions to stimulate new conidial production. Significant cumulative conidial production was observed after the second harvest.

Associated Publications: Rodríguez-García CM, Ruiz-Ruiz JC, Peraza-Echeverría L, Peraza-Sánchez SR, Torres-Tapia LW, Pérez-Brito D, Tapia-Tussell R, Herrera-Chalé FG, Segura-Campos MR, Quijano-Ramayo A, Ramón-Sierra JM, Ortiz-Vázquez E (2019) Antioxidant, antihypertensive, anti-hyperglycemic, and antimicrobial activity of aqueous extracts from twelve native plants of the Yucatan coast. PLoS ONE 14(3): e0213493. doi: 10.1371/journal.pone.0213493

Affiliations: Centro de Investigación Científica de Yucatán, Centro de Investigación Científica de Yucatán, Centro de Investigación Científica de Yucatán

External URL: https://link.springer.com/article/10.1071/AP08042

Version: 1

Publication Date: 2018

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