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Protocol Name
Endo F1 Protocol for Deglycosylating Glycoproteins
DOI:DOI:10.17504/protocols.io.sebeban RRID Copied  
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Mike Gibson 2018. Endo F1 Protocol for Deglycosylating Glycoproteins. protocols.io https://dx.doi.org/10.17504/protocols.io.sebeban
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.sebeban

Authors: Mike Gibson

Summary: Endo F1 cleaves Asparagine-linked high mannose or hybrid oligosaccharides. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact.

Affiliations: QA-Bio Inc

External URL: https://www.qa-bio.com/product/endo-f1/

Version: 1

Publication Date: 2018

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