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Caio Maximino, Jeisiane Souza de Oliveira, Monica Gomes Lima-Maximino 2018. Optimized protocol for quantification of nitrite levels in brain and head kidney tissue samples in adult zebrafish. protocols.io https://dx.doi.org/10.17504/protocols.io.sabeaanCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.sabeaan
Authors: Caio Maximino, Jeisiane Souza de Oliveira, Monica Gomes Lima-Maximino
Group: Fish behavior and physiology
Summary: Zebrafish, and other small teleosts, are used as experimental models to evaluate human pathologies, including those linked to oxidative and nitrosative stress, inflammation, and nitric oxide signaling. The protocol presents an optimized technique to quantify nitrite levels, in zebrafish tissues, focusing on the brain and head kidney. The protocol is based on the classical Griess diazotization reaction (Griess, 1858) method.
Affiliations: Universidade Federal do Sul e Sudeste do Pará, Universidade do Estado do Pará, Universidade do Estado do Pará
Version: 1
Publication Date: 2018
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