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Andrew Potter 2018. Adult mouse liver dissociation (on ice). protocols.io https://dx.doi.org/10.17504/protocols.io.r8id9ueCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.r8id9ue
Authors: Andrew Potter
Group: Human Cell Atlas Method Development Community
Summary: This protocol is used to dissociate adult (10 week) CD-1 mouse liver "on ice", preserving authentic gene expression profiles. The first layer consists of 3.5 hours rotation at 4 °C in 0.25% trypsin, and the second layer consists of 15 min. in bacillus licheniformis, with trituration on ice. The yield is 5000 cells/mg with 97% viability (as measured by trypan blue).
Affiliations: University of Cincinnati
Version: 1
Publication Date: 2018
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