Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Pringle Lab, Christian Renicke 2018. Embedding living larvae in low-melting agarose for imaging and recovery thereafter. protocols.io https://dx.doi.org/10.17504/protocols.io.qxbdxinCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.qxbdxin
Authors: Pringle Lab, Christian Renicke
Group: Aiptasia-Symbiodiniaceae Model System
Summary: This protocol describes how to use agarose with a low gelling point (27-30°C; often called low-melting agarose) to immobilize Aiptasia larvae for microscopy.Larvae stay alive and in one place for prolonged periods of several hours but continue to mainly spin around the longitudal axis. After imaging, larvae can easily be retrieved from the agarose and transferred back to artificial sea water.
Affiliations: Stanford University, Stanford University
Version: 1
Publication Date: 2018
Expand AllComing soon.
Check
for resource mentions.
Coming soon.
Source:
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
